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Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method
Joint Authors
Do, Anh Ngoc
Le, Tran-Anh
Tuan, Le Quoc
Bac, Nguyen Duy
Tien, Tran Viet
Phuong, Vu Thi Binh
Duong, Tran Thanh
Luc, Nguyen Khac
Quang, Nguyen Ba
Source
Journal of Parasitology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Fascioliasis—a disease caused by Fasciola spp.
(Platyhelminthes: Trematoda: Digenea)—is considered as the most important helminthic infection of bovine, sheep, and buffalo in Vietnam.
The aim of this study is to detect the genotype of Fasciola spp.
isolated from bovine and buffalo in the Nghe An province, central Vietnam, using PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1).
Adult Fasciola spp.
were isolated from bile ducts of bovine and buffalo in Nghe An province, Vietnam.
Overall, 96 adult flukes from livers of slaughtered animals were collected from abattoirs of different areas.
They included 7 samples from infected bovine and 89 samples from infected buffalo.
96/96 samples were identified as Fasciola species by ITS1 of rDNA.
In this study, a PCR-RFLP method was used to distinguish between F.
hepatica and F.
gigantica in ITS1 of rDNA (680 bp) with RsaI restriction enzyme.
RFLP pattern with RsaI produced a consistent pattern of 360, 100, and 60 bp fragments in F.
hepatica, whereas F.
gigantica worms had a profile of 360, 170, and 60 bp in size, respectively.
The results showed that using PCR-RFLP based on the first internal transcribed spacers (ITS1) of the ribosomal RNA revealed that 93 out of 96 isolates were of Fasciola gigantica type, whereas three isolates presented an intermediate Fasciola.
In the present study, F.
gigantica and intermediate form were coexisting in bovine and buffalo in the Nghe An province of central Vietnam, whereas F.
hepatica was not detected.
American Psychological Association (APA)
Do, Anh Ngoc& Le, Tran-Anh& Tuan, Le Quoc& Bac, Nguyen Duy& Tien, Tran Viet& Phuong, Vu Thi Binh…[et al.]. 2018. Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method. Journal of Parasitology Research،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1197572
Modern Language Association (MLA)
Do, Anh Ngoc…[et al.]. Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method. Journal of Parasitology Research No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1197572
American Medical Association (AMA)
Do, Anh Ngoc& Le, Tran-Anh& Tuan, Le Quoc& Bac, Nguyen Duy& Tien, Tran Viet& Phuong, Vu Thi Binh…[et al.]. Identification of Fasciola Species Isolates from Nghe An Province, Vietnam, Based on ITS1 Sequence of Ribosomal DNA Using a Simple PCR-RFLP Method. Journal of Parasitology Research. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1197572
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197572