Effect of Red Light-Emitting Diodes Irradiation on Hemoglobin for Potential Hypertension Treatment Based on Confocal Micro-Raman Spectroscopy

Abstract EN

Red light-emitting diodes (LED) were used to irradiate the isolated hypertension hemoglobin (Hb) and Raman spectra difference was recorded using confocal micro-Raman spectroscopy.

Differences were observed between the controlled and irradiated Hb by comparing the spectra records.

The Raman spectrum at the 1399 cm−1 band decreased following prolonged LED irradiation.

The intensity of the 1639 cm−1 band decreased dramatically in the first five minutes and then gradually increased in a time-dependent manner.

This observation indicated that LED irradiation increased the ability of oxygen binding in Hb.

The appearance of the heme aggregation band at 1399 cm−1, in addition to the oxygen marker band at 1639 cm−1, indicated that, in our study, 30 min of irradiation with 15.0 mW was suitable for inhibiting heme aggregation and enhancing the oxygen-carrying capacity of Hb.

Principal component analysis showed a one-to-one relationship between irradiated Hb at different time points and the corresponding Raman spectra.

Our approach could be used to analyze the hemoglobin from patients with confocal micro-Raman spectroscopy and is helpful for developing new nondrug hypertension therapy.