Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3
Joint Authors
Michel, Milton Domingos
Pupo, Yasmine Mendes
Bernardo, Cintia Fernanda de Freitas
de Souza, Francielly Fernanda de Freitas A.
Ribeiro, Camila Nunes de Morais
Germano, Sandro
Maluf, Daniela Florencio
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Information Technology and Computer Science
Abstract EN
The aim of this study was to evaluate in fibroblast cultures the direct cytotoxic effects of etch-and-rinse, self-etch, and universal adhesive systems.
The sterile glass cover slips (n=3) were then immersed in culture medium to obtain the eluates for the experimental groups: (1) Adper™ Single Bond 2; (2) Ambar; (3) Adper™ Scotchbond™ Multi-Purpose; (4) Scotchbond™ Universal; (5) Ambar Universal; and (6) OptiBond All-In-One.
As a negative control, sterile glass cover slips were immersed in culture medium only.
After 24 h, the eluate obtained was applied on fibroblast culture.
Cell viability and cell morphology were evaluated by MTT assay and SEM, respectively.
Data were analyzed by Kruskal–Wallis and Mann–Whitney tests (α=0.05).
All adhesive systems except universal reduced cell viability in 3T3 cells to between 26.04% and 56.57%, and Scotchbond Universal and Ambar Universal reduced cell viability to 2.13% and 3.57%, respectively, when compared to the negative control.
Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed.
In conclusion, improvements in universal adhesive system formulations and their mechanisms of action are not accompanied by increased toxicity compared with those in other systems, warranting commitment to the use of these dentin-pulp complexes.
American Psychological Association (APA)
Pupo, Yasmine Mendes& Bernardo, Cintia Fernanda de Freitas& de Souza, Francielly Fernanda de Freitas A.& Michel, Milton Domingos& Ribeiro, Camila Nunes de Morais& Germano, Sandro…[et al.]. 2017. Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3. Scanning،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197997
Modern Language Association (MLA)
Pupo, Yasmine Mendes…[et al.]. Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3. Scanning No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1197997
American Medical Association (AMA)
Pupo, Yasmine Mendes& Bernardo, Cintia Fernanda de Freitas& de Souza, Francielly Fernanda de Freitas A.& Michel, Milton Domingos& Ribeiro, Camila Nunes de Morais& Germano, Sandro…[et al.]. Cytotoxicity of Etch-and-Rinse, Self-Etch, and Universal Dental Adhesive Systems in Fibroblast Cell Line 3T3. Scanning. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197997