From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells
Joint Authors
Girard, Stéphane D.
Virard, Isabelle
Lacassagne, Emmanuelle
Paumier, Jean-Michel
Lahlou, Hanae
Jabes, Françoise
Molino, Yves
Stephan, Delphine
Baranger, Kevin
Belghazi, Maya
Deveze, Arnaud
Khrestchatisky, Michel
Nivet, Emmanuel
Roman, François S.
Féron, François
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-18
Country of Publication
Egypt
No. of Pages
17
Abstract EN
Stem cell-based therapies critically rely on selective cell migration toward pathological or injured areas.
We previously demonstrated that human olfactory ectomesenchymal stem cells (OE-MSCs), derived from an adult olfactory lamina propria, migrate specifically toward an injured mouse hippocampus after transplantation in the cerebrospinal fluid and promote functional recoveries.
However, the mechanisms controlling their recruitment and homing remain elusive.
Using an in vitro model of blood-brain barrier (BBB) and secretome analysis, we observed that OE-MSCs produce numerous proteins allowing them to cross the endothelial wall.
Then, pan-genomic DNA microarrays identified signaling molecules that lesioned mouse hippocampus overexpressed.
Among the most upregulated cytokines, both recombinant SPP1/osteopontin and CCL2/MCP-1 stimulate OE-MSC migration whereas only CCL2 exerts a chemotactic effect.
Additionally, OE-MSCs express SPP1 receptors but not the CCL2 cognate receptor, suggesting a CCR2-independent pathway through other CCR receptors.
These results confirm that OE-MSCs can be attracted by chemotactic cytokines overexpressed in inflamed areas and demonstrate that CCL2 is an important factor that could promote OE-MSC engraftment, suggesting improvement for future clinical trials.
American Psychological Association (APA)
Girard, Stéphane D.& Virard, Isabelle& Lacassagne, Emmanuelle& Paumier, Jean-Michel& Lahlou, Hanae& Jabes, Françoise…[et al.]. 2017. From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1200717
Modern Language Association (MLA)
Girard, Stéphane D.…[et al.]. From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells. Stem Cells International No. 2017 (2017), pp.1-17.
https://search.emarefa.net/detail/BIM-1200717
American Medical Association (AMA)
Girard, Stéphane D.& Virard, Isabelle& Lacassagne, Emmanuelle& Paumier, Jean-Michel& Lahlou, Hanae& Jabes, Françoise…[et al.]. From Blood to Lesioned Brain: An In Vitro Study on Migration Mechanisms of Human Nasal Olfactory Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1200717
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1200717