![](/images/graphics-bg.png)
Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization
Joint Authors
Schenke-Layland, Katja
Brauchle, Eva
Carvajal Berrio, Daniel
Rieger, Melanie
Reinert, Siegmar
Alexander, Dorothea
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-23
Country of Publication
Egypt
No. of Pages
12
Abstract EN
To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies.
In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1+ osteoprogenitors derived from the jaw periosteum.
In this study, the in vitro formation of bone-specific matrix by MSCA-1+ jaw periosteal cells (JPCs, 3 donors) was assessed and compared under serum-free and serum-containing media conditions using the marker-free Raman spectroscopy.
Based on a standard fluorescence assay, JPCs from one patient were not able to mineralize under serum-containing culture conditions, whereas the other cells showed similar mineralization levels under both conditions.
Raman spectra from mineralizing MSCA-1+ JPCs revealed higher levels of hydroxyapatite formation and higher mineral to matrix ratios under serum-free culture conditions.
Higher carbonate to phosphate ratios and higher crystallinity in JPCs cultured under serum-containing conditions indicated immature bone formation.
Due to reduced collagen production under serum-free conditions, we obtained significant differences in collagen maturity and proline to hydroxyproline ratios compared to serum-free conditions.
We conclude that Raman spectroscopy is a useful tool for the assessment and noninvasive monitoring of in vitro mineralization of osteoprogenitor cells.
Further studies should extend this knowledge and improve JPC mineralization by optimizing culture conditions.
American Psychological Association (APA)
Brauchle, Eva& Carvajal Berrio, Daniel& Rieger, Melanie& Schenke-Layland, Katja& Reinert, Siegmar& Alexander, Dorothea. 2017. Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1200788
Modern Language Association (MLA)
Brauchle, Eva…[et al.]. Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1200788
American Medical Association (AMA)
Brauchle, Eva& Carvajal Berrio, Daniel& Rieger, Melanie& Schenke-Layland, Katja& Reinert, Siegmar& Alexander, Dorothea. Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1200788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1200788