miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells
Joint Authors
Chen, Zhong-Yan
Chen, Fei
Cao, Nan
Zhou, Zhi-Wen
Yang, Huang-Tian
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-05
Country of Publication
Egypt
No. of Pages
10
Abstract EN
MicroRNAs (miRNAs) play important roles in cell fate decisions.
However, the miRNAs and their targets involved in the regulation of cardiac lineage specification are largely unexplored.
Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs).
With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs.
Ectopic expression and inhibition of miR-142-3p did not alter the characteristics of undifferentiated ESCs, whereas ectopic expression of miR-142-3p impaired cardiomyocyte formation.
In addition, ectopic expression of miR-142-3p inhibited the expression of a cardiac mesodermal marker gene Mesp1 and downstream cardiac transcription factors Nkx2.5, Tbx5, and Mef2c but not the expression of three germ layer-specific genes.
We further demonstrated that miR-142-3p targeted the 3′-untranslated region of Mef2c.
These results reveal miR-142-3p as an important regulator of early cardiomyocyte differentiation.
Our findings provide new knowledge for further understanding of roles and mechanisms of miRNAs as critical regulators of cardiomyocyte differentiation.
American Psychological Association (APA)
Chen, Zhong-Yan& Chen, Fei& Cao, Nan& Zhou, Zhi-Wen& Yang, Huang-Tian. 2017. miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1200847
Modern Language Association (MLA)
Chen, Zhong-Yan…[et al.]. miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells. Stem Cells International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1200847
American Medical Association (AMA)
Chen, Zhong-Yan& Chen, Fei& Cao, Nan& Zhou, Zhi-Wen& Yang, Huang-Tian. miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1200847
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1200847