Simple Meets Single: The Application of CRISPRCas9 in Haploid Embryonic Stem Cells
Joint Authors
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-03
Country of Publication
Egypt
No. of Pages
6
Abstract EN
The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening.
However, the diploid status of most mammalian cells restricts the application of CRISPR/Cas9 in genetic screening.
Mammalian haploid embryonic stem cells (haESCs) have only one set of chromosomes per cell, avoiding the issue of heterozygous recessive mutations in diploid cells.
Thus, the combination of haESCs and CRISPR/Cas9 facilitates the generation of genome-wide knockout cell libraries for genetic screening.
Here, we review recent progress in CRISPR/Cas9 and haPSCs and discuss their applications in genetic screening.
American Psychological Association (APA)
Yin, Zixi& Chen, Lingyi. 2017. Simple Meets Single: The Application of CRISPRCas9 in Haploid Embryonic Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1201019
Modern Language Association (MLA)
Yin, Zixi& Chen, Lingyi. Simple Meets Single: The Application of CRISPRCas9 in Haploid Embryonic Stem Cells. Stem Cells International No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1201019
American Medical Association (AMA)
Yin, Zixi& Chen, Lingyi. Simple Meets Single: The Application of CRISPRCas9 in Haploid Embryonic Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1201019
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201019