Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium
Joint Authors
Yu, Shuai
Zhang, Pengfei
Dong, Wuzi
Zeng, Wenxian
Pan, Chuanying
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-24
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Stem Leydig cells (SLCs), located in the testicular interstitial compartment in the mammalian testes, are capable of differentiating to testosterone-synthesizing Leydig cells (LCs), thus providing a new strategy for treating testosterone deficiency.
However, no previous reports have identified and cultured SLCs derived from the pig.
The aim of the current study was to isolate, identify, and culture SLCs from pigs.
Haematoxylin and eosin staining and immunochemical analysis showed that SLCs were present and that PDGFRα was mainly expressed in the pig testicular interstitium, indicating that PDGFRα was a marker for SLCs in the neonatal pig.
In addition, reverse transcription-PCR results showed that SLC markers were expressed in primary isolated LCs, indicating that they were putative SLCs.
The putative SLCs were subsequently cultured with a testicular fluid of piglets (pTF) medium.
Clones formed after 7 days and the cells expressed PDGFRα.
However, no clones grew in the absence of pTF, but the cells expressed CYP17A1, indicating that pTF could sustain the features of porcine SLCs.
To summarize, we isolated porcine SLCs and identified their basic characteristics.
Taken together, these results may help lay the foundation for research in the clinical application of porcine SLCs.
American Psychological Association (APA)
Yu, Shuai& Zhang, Pengfei& Dong, Wuzi& Zeng, Wenxian& Pan, Chuanying. 2017. Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium. Stem Cells International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1201039
Modern Language Association (MLA)
Yu, Shuai…[et al.]. Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium. Stem Cells International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1201039
American Medical Association (AMA)
Yu, Shuai& Zhang, Pengfei& Dong, Wuzi& Zeng, Wenxian& Pan, Chuanying. Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1201039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201039