Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic
Joint Authors
Krešić, Nina
Šimić, Ivana
Lojkić, Ivana
Bedeković, Tomislav
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-26
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Although canine adipose derived stem cells (cASCs) morphology characteristics and differentiation ability are well documented, transcriptome alterations of undifferentiated cASCs during ex vivo cultivation remain unknown.
Here we demonstrate, for the first time, the transcriptome composition of isolated cASCs in undifferentiated state originating from six donors.
Transcriptome changes were monitored during ex vivo cultivation between passage 3 (P3) and P5, which are mostly used in therapy.
Influence of donors’ age in given passage number on transcriptome composition was also investigated.
Cultivation from P3 to P5 resulted in 16 differentially expressed genes with cooverexpression of pluripotency and self-renewal transcription factors genes SOX2 and POU5F1 dominant in old donors’ cells.
Furthermore, cASCs demonstrated upregulation of IL-6 in young and old donors’ cells.
In addition, ex vivo cultivation of cASCs revealed well-known morphological alterations accompanied with decrease in expression of CD90 and CD44 markers in P4 and higher monitored by flow cytometry and successful osteo- and chondrodifferentiation but inefficient adipodifferentiation in P3.
Our results revealed the impact of ex vivo cultivation on nature of cells.
Correlation of transcriptome changes with secretome composition is needed and its further impact on therapeutic potential of cASCs remains to be evaluated in clinical trials.
American Psychological Association (APA)
Krešić, Nina& Šimić, Ivana& Lojkić, Ivana& Bedeković, Tomislav. 2017. Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic. Stem Cells International،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1201521
Modern Language Association (MLA)
Krešić, Nina…[et al.]. Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic. Stem Cells International No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1201521
American Medical Association (AMA)
Krešić, Nina& Šimić, Ivana& Lojkić, Ivana& Bedeković, Tomislav. Canine Adipose Derived Mesenchymal Stem Cells Transcriptome Composition Alterations: A Step towards Standardizing Therapeutic. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1201521
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201521