Optogenetic Inhibition of Striatal Neuronal Activity Improves the Survival of Transplanted Neural Stem Cells and Neurological Outcomes after Ischemic Stroke in Mice
Joint Authors
Yang, Guo-Yuan
Lu, Yifan
Jiang, Lu
Li, Wanlu
Qu, Meijie
Song, Yaying
He, Xiaosong
Zhang, Zhijun
Wang, Yongting
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-14
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Neural stem cell (NSC) transplantation is a promising treatment to improve the recovery after brain ischemia.
However, how the survival, proliferation, migration, and differentiation of implanted NSC are influenced by endogenous neuronal activity remains unclear.
In this work, we used optogenetic techniques to control the activity of striatal neurons and investigated how their activity affected the survival and migration of transplanted NSCs and overall neurological outcome after ischemic stroke.
NSCs cultured from transgenic mice expressing fluorescent protein were transplanted into the peri-infarct region of the striatum after transient middle cerebral artery occlusion (tMCAO) surgery.
The striatal neurons were excited or inhibited for 15 minutes daily via implanted optical fiber after tMCAO.
The results revealed that mice which received NSC transplantation and optogenetic inhibition had smaller brain infarct volume and increased NSC migration compared to the NSC alone or PBS group (p<0.05).
In contrast, mice which received NSC transplantation and optogenetic excitation showed no difference in infarct volume and neurological behavior improvement compared to the PBS control group.
In vitro experiments further revealed that the conditioned media from excited GABAergic neurons reduced NSC viability through paracrine mechanisms.
Conclusion.
Optogenetic inhibition of striatal neuronal activity further improved neurological recovery after NSC transplantation at the subacute phase after brain ischemia.
American Psychological Association (APA)
Lu, Yifan& Jiang, Lu& Li, Wanlu& Qu, Meijie& Song, Yaying& He, Xiaosong…[et al.]. 2017. Optogenetic Inhibition of Striatal Neuronal Activity Improves the Survival of Transplanted Neural Stem Cells and Neurological Outcomes after Ischemic Stroke in Mice. Stem Cells International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201567
Modern Language Association (MLA)
Lu, Yifan…[et al.]. Optogenetic Inhibition of Striatal Neuronal Activity Improves the Survival of Transplanted Neural Stem Cells and Neurological Outcomes after Ischemic Stroke in Mice. Stem Cells International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1201567
American Medical Association (AMA)
Lu, Yifan& Jiang, Lu& Li, Wanlu& Qu, Meijie& Song, Yaying& He, Xiaosong…[et al.]. Optogenetic Inhibition of Striatal Neuronal Activity Improves the Survival of Transplanted Neural Stem Cells and Neurological Outcomes after Ischemic Stroke in Mice. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201567
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201567