TNF-α Induced the Enhanced Apoptosis of Mesenchymal Stem Cells in Ankylosing Spondylitis by Overexpressing TRAIL-R2
Joint Authors
Ouyang, Yi
Li, Yuxi
Wang, Peng
Xie, Zhongyu
Wu, Yanfeng
Shen, Huiyong
Su, Hongjun
Li, Jinteng
Deng, Wen
Liu, Zhenhua
Gao, Liangbin
Cen, Shuizhong
Wu, Xiaohua
Wang, Le
Wang, Shan
Li, Deng
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-15
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Ankylosing spondylitis (AS) is an autoimmune disease with unknown etiology.
Dysregulated mesenchymal stem cells (MSCs) apoptosis may contribute to the pathogenesis of autoimmune diseases.
However, apoptosis of MSCs from patients with AS (ASMSCs) has not been investigated yet.
The present study aims to assess the apoptosis of bone marrow-derived ASMSCs and to investigate the underlying mechanisms of altered ASMSCs apoptosis.
We successfully induced the apoptosis of ASMSCs and MSCs from healthy donors (HDMSCs) using the combination of tumor necrosis factor alpha (TNF-α) and cycloheximide (CHX).
We found that ASMSCs treated with TNF-α and CHX showed higher apoptosis levels compared to HDMSCs.
During apoptosis, ASMSCs expressed significantly more TRAIL-R2, which activated both the death receptor pathway and mitochondria pathway by increasing the expression of FADD, cleaved caspase-8, cytosolic cytochrome C, and cleaved caspase-3.
Inhibiting TRAIL-R2 expression using shRNA eliminated the apoptosis differences between HDMSCs and ASMSCs by partially reducing ASMSCs apoptosis but minimally affecting that of HDMSCs.
Furthermore, the expression of FADD, cleaved caspase-8, cytosolic cytochrome C, and cleaved caspase-3 were comparable between HDMSCs and ASMSCs after TRAIL-R2 inhibition.
These results indicated that increased TRAIL-R2 expression results in enhanced ASMSCs apoptosis and may contribute to AS pathogenesis.
American Psychological Association (APA)
Liu, Zhenhua& Gao, Liangbin& Wang, Peng& Xie, Zhongyu& Cen, Shuizhong& Li, Yuxi…[et al.]. 2017. TNF-α Induced the Enhanced Apoptosis of Mesenchymal Stem Cells in Ankylosing Spondylitis by Overexpressing TRAIL-R2. Stem Cells International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1201582
Modern Language Association (MLA)
Liu, Zhenhua…[et al.]. TNF-α Induced the Enhanced Apoptosis of Mesenchymal Stem Cells in Ankylosing Spondylitis by Overexpressing TRAIL-R2. Stem Cells International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1201582
American Medical Association (AMA)
Liu, Zhenhua& Gao, Liangbin& Wang, Peng& Xie, Zhongyu& Cen, Shuizhong& Li, Yuxi…[et al.]. TNF-α Induced the Enhanced Apoptosis of Mesenchymal Stem Cells in Ankylosing Spondylitis by Overexpressing TRAIL-R2. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1201582
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201582