iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression
Joint Authors
Qu, Shanshan
Zhang, Jiping
Huang, Yong
Zhong, Zheng
Zhang, Jialing
Zhang, Zhinan
Yao, Zengyu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-02
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Hippocampal atrophy is one of the key changes in the brain implicated in the biology of depression.
However, the precise molecular mechanism remains poorly understood due to a lack of biomarkers.
In this research, we used behavioral experiments to evaluate anxiety and anhedonia levels in depressed rats using chronic unpredictable mild stress (CUMS) modeling.
We also used isobaric tag for relative and absolute quantitation (iTRAQ) to identify the differentially expressed hippocampal proteins between depressed and normal rats.
Bioinformatics analyses were also performed for a better understanding.
The results showed that CUMS rats had higher anxiety and anhedonia levels than control rats, along with hippocampal lesions.
Through iTRAQ and bioinformatics analyses, we found that ribosome proteins were significantly downregulated and Ras proteins exhibited a mixed change in the hippocampus of depressed rats.
These findings suggest that the expression of hippocampal ribosome lesions and Ras proteins is significantly different in depressed rats than in control rats, providing new insights into the neurobiology of depression.
American Psychological Association (APA)
Zhang, Jialing& Zhang, Zhinan& Zhang, Jiping& Zhong, Zheng& Yao, Zengyu& Qu, Shanshan…[et al.]. 2019. iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression. Neural Plasticity،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1201602
Modern Language Association (MLA)
Zhang, Jialing…[et al.]. iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression. Neural Plasticity No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1201602
American Medical Association (AMA)
Zhang, Jialing& Zhang, Zhinan& Zhang, Jiping& Zhong, Zheng& Yao, Zengyu& Qu, Shanshan…[et al.]. iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression. Neural Plasticity. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1201602
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201602