Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage
Joint Authors
Mazzon, Emanuela
Caputi, Sergio
Marchisio, Marco
Diomede, Francesca
Rajan, Thangavelu Soundara
D’Aurora, Marco
Merciaro, Ilaria
Gatta, Valentina
Trubiani, Oriana
Muttini, Aurelio
Bramanti, Placido
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-02
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Background.
Neural crest-derived mesenchymal stem cells (MSCs) from human oral tissues possess immunomodulatory and regenerative properties and are emerging as a potential therapeutic tool to treat diverse diseases, such as multiple sclerosis, myocardial infarction, and connective tissue damages.
In addition to cell-surface antigens, dental MSCs express embryonic stem cell markers as neural crest cells originate from the ectoderm layer.
In vitro passages may eventually modify these embryonic marker expressions and other stemness properties, including proliferation.
In the present study, we have investigated the expression of proteins involved in cell proliferation/senescence and embryonic stem cell markers during early (passage 2) and late passages (passage 15) in MSCs obtained from human gingiva, periodontal, and dental pulp tissues.
Methods.
Cell proliferation assay, beta galactosidase staining, immunocytochemistry, and real-time PCR techniques were applied.
Results.
Cell proliferation assay showed no difference between early and late passages while senescence markers p16 and p21 were considerably increased in late passage.
Embryonic stem cell markers including SKIL, MEIS1, and JARID2 were differentially modulated between P2 and P15 cells.
Discussion.
Our results suggest that the presence of embryonic and proliferation markers even in late passage may potentially endorse the application of dental-derived MSCs in stem cell therapy-based clinical trials.
American Psychological Association (APA)
Diomede, Francesca& Rajan, Thangavelu Soundara& Gatta, Valentina& D’Aurora, Marco& Merciaro, Ilaria& Marchisio, Marco…[et al.]. 2017. Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage. Stem Cells International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1201779
Modern Language Association (MLA)
Diomede, Francesca…[et al.]. Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage. Stem Cells International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1201779
American Medical Association (AMA)
Diomede, Francesca& Rajan, Thangavelu Soundara& Gatta, Valentina& D’Aurora, Marco& Merciaro, Ilaria& Marchisio, Marco…[et al.]. Stemness Maintenance Properties in Human Oral Stem Cells after Long-Term Passage. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1201779
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201779