Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections
Joint Authors
Adjaye, James
Spitzhorn, Lucas-Sebastian
Rahman, Md Shaifur
Schwindt, Laura
Ho, Huyen-Tran
Wruck, Wasco
Bohndorf, Martina
Wehrmeyer, Silke
Ncube, Audrey
Hagenbeck, Carsten
Balan, Percy
Fehm, T.
Beyer, I.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-31
Country of Publication
Egypt
No. of Pages
15
Abstract EN
Human amniotic fluid cells are immune-privileged with low immunogenicity and anti-inflammatory properties.
They are able to self-renew, are highly proliferative, and have a broad differentiation potential, making them amenable for cell-based therapies.
Amniotic fluid (AF) is routinely obtained via amniocentesis and contains heterogeneous populations of foetal-derived progenitor cells including mesenchymal stem cells (MSCs).
In this study, we isolated human MSCs from AF (AF-MSCs) obtained during Caesarean sections (C-sections) and characterized them.
These AF-MSCs showed typical MSC characteristics such as morphology, in vitro differentiation potential, surface marker expression, and secreted factors.
Besides vimentin and the stem cell marker CD133, subpopulations of AF-MSCs expressed pluripotency-associated markers such as SSEA4, c-Kit, TRA-1-60, and TRA-1-81.
The secretome and related gene ontology (GO) terms underline their immune modulatory properties.
Furthermore, transcriptome analyses revealed similarities with native foetal bone marrow-derived MSCs.
Significant KEGG pathways as well as GO terms are mostly related to immune function, embryonic skeletal system, and TGFβ-signalling.
An AF-MSC-enriched gene set included putative AF-MSC markers PSG5, EMX-2, and EVR-3.
In essence, C-section-derived AF-MSCs can be routinely obtained and are amenable for personalized cell therapies and disease modelling.
American Psychological Association (APA)
Spitzhorn, Lucas-Sebastian& Rahman, Md Shaifur& Schwindt, Laura& Ho, Huyen-Tran& Wruck, Wasco& Bohndorf, Martina…[et al.]. 2017. Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections. Stem Cells International،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1201873
Modern Language Association (MLA)
Spitzhorn, Lucas-Sebastian…[et al.]. Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections. Stem Cells International No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1201873
American Medical Association (AMA)
Spitzhorn, Lucas-Sebastian& Rahman, Md Shaifur& Schwindt, Laura& Ho, Huyen-Tran& Wruck, Wasco& Bohndorf, Martina…[et al.]. Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1201873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201873