Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells
Joint Authors
Cugat, Ramón
García-Balletbó, Montserrat
Mellado-López, Maravillas
Griffeth, Richard J
Moreno-Manzano, Victoria
Meseguer-Ripolles, Jose
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-15
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Adipose-derived stem cells (ASCs) are a promising therapeutic alternative for tissue repair in various clinical applications.
However, restrictive cell survival, differential tissue integration, and undirected cell differentiation after transplantation in a hostile microenvironment are complications that require refinement.
Plasma rich in growth factors (PRGF) from platelet-rich plasma favors human and canine ASC survival, proliferation, and delaying human ASC senescence and autophagocytosis in comparison with serum-containing cultures.
In addition, canine and human-derived ASCs efficiently differentiate into osteocytes, adipocytes, or chondrocytes in the presence of PRGF.
PRGF treatment induces phosphorylation of AKT preventing ASC death induced by lethal concentrations of hydrogen peroxide.
Indeed, AKT inhibition abolished the PRGF apoptosis prevention in ASC exposed to 100 μM of hydrogen peroxide.
Here, we show that canine ASCs respond to PRGF stimulus similarly to the human cells regarding cell survival and differentiation postulating the use of dogs as a suitable translational model.
Overall, PRGF would be employed as a serum substitute for mesenchymal stem cell amplification to improve cell differentiation and as a preconditioning agent to prevent oxidative cell death.
American Psychological Association (APA)
Mellado-López, Maravillas& Griffeth, Richard J& Meseguer-Ripolles, Jose& Cugat, Ramón& García-Balletbó, Montserrat& Moreno-Manzano, Victoria. 2017. Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201882
Modern Language Association (MLA)
Mellado-López, Maravillas…[et al.]. Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells. Stem Cells International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1201882
American Medical Association (AMA)
Mellado-López, Maravillas& Griffeth, Richard J& Meseguer-Ripolles, Jose& Cugat, Ramón& García-Balletbó, Montserrat& Moreno-Manzano, Victoria. Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201882
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201882