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hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System
Joint Authors
An, Ke
Liu, Hui-ping
Zhong, Xiao-long
Deng, David Y. B.
Zhang, Jing-jun
Liu, Zhi-heng
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-11
Country of Publication
Egypt
No. of Pages
11
Abstract EN
The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management.
Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner.
The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain.
American Psychological Association (APA)
An, Ke& Liu, Hui-ping& Zhong, Xiao-long& Deng, David Y. B.& Zhang, Jing-jun& Liu, Zhi-heng. 2017. hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System. Stem Cells International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201911
Modern Language Association (MLA)
An, Ke…[et al.]. hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System. Stem Cells International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1201911
American Medical Association (AMA)
An, Ke& Liu, Hui-ping& Zhong, Xiao-long& Deng, David Y. B.& Zhang, Jing-jun& Liu, Zhi-heng. hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201911
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201911