Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele
Joint Authors
Deprest, Jan
Sampaolesi, Maurilio
Sahakyan, Vardine
Pozzo, Enrico
Duelen, Robin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-13
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Myelomeningocele (MMC) is a severe type of neural tube defect (NTD), in which the backbone and spinal canal do not close completely during early embryonic development.
This condition results in serious morbidity and increased mortality after birth.
Folic acid significantly reduces, and conversely, folate antagonist methotrexate (MTX) and valproic acid (VPA) increase the occurrence of NTDs, including MMC.
How these pharmacological agents exactly influence the early neurulation process is still largely unclear.
Here, we characterized human amniotic fluid-derived stem cells (AFSCs) from prenatally diagnosed MMC and observed an effect of MTX and VPA administration on the early neural differentiation process.
We found that MMC-derived AFSCs highly expressed early neural and radial glial genes that were negatively affected by MTX and VPA exposure.
In conclusion, we setup a human cell model of MMC to study early neurogenesis and for drug screening purposes.
We also proposed the detection of early neural gene expression in AFSCs as an additional MMC diagnostic tool.
American Psychological Association (APA)
Sahakyan, Vardine& Pozzo, Enrico& Duelen, Robin& Deprest, Jan& Sampaolesi, Maurilio. 2017. Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele. Stem Cells International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1201915
Modern Language Association (MLA)
Sahakyan, Vardine…[et al.]. Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele. Stem Cells International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1201915
American Medical Association (AMA)
Sahakyan, Vardine& Pozzo, Enrico& Duelen, Robin& Deprest, Jan& Sampaolesi, Maurilio. Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1201915
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201915