Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-βERKp90RSK Signalling
Joint Authors
Lu, Lungen
Li, Fei
Ma, Zhenzeng
Liu, Heng
Zhang, Qidi
Cai, Xiaobo
Qu, Ying
Xu, Mingyi
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-27
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Y-box protein-1 (YB-1) is a highly conserved transcription factor that is involved in multiple biological processes via transcriptional regulation of several genes, including p53, cyclin D1, and EGFR.
YB-1 has been reported to be overexpressed in injured livers.
This study aims to explore the functions of YB-1 in hepatic progenitor cells (HPCs).
Herein, chromatin immunoprecipitation sequencing (ChIP-sequencing) and RNA-sequencing assays identified that YB-1 participated in the biological adhesion process and ECM-receptor interactions in HPCs.
Further study demonstrated that YB-1 modulated the expression of extracellular matrix components in HPCs.
ChIP-sequencing assays established that PDGFR-β was a target gene of YB-1, and luciferase reporter assays confirmed that YB-1 negatively regulated PDGFR-β promoter activity in HPCs.
In addition, PDGFR-β can regulate the expression of collagen I through ERK/p90RSK signalling, and disruption of the signalling pathway with a PDGFR-β inhibitor or ERK1/2 inhibitor abolished the regulatory effect of PDGFR-β on collagen I expression in HPCs.
Conclusively, YB-1 can modulate the expression of collagen I in HPCs via direct binding to the PDGFR-β promoter, negatively regulating its expression.
In addition, the ERK/p90RSK axis serves as the downstream signalling pathway of PDGFR-β.
American Psychological Association (APA)
Li, Fei& Ma, Zhenzeng& Liu, Heng& Zhang, Qidi& Cai, Xiaobo& Qu, Ying…[et al.]. 2017. Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-βERKp90RSK Signalling. Stem Cells International،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201953
Modern Language Association (MLA)
Li, Fei…[et al.]. Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-βERKp90RSK Signalling. Stem Cells International No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1201953
American Medical Association (AMA)
Li, Fei& Ma, Zhenzeng& Liu, Heng& Zhang, Qidi& Cai, Xiaobo& Qu, Ying…[et al.]. Y-box Protein-1 Regulates the Expression of Collagen I in Hepatic Progenitor Cells via PDGFR-βERKp90RSK Signalling. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1201953
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201953