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Influence of Different ECM-Like Hydrogels on Neurite Outgrowth Induced by Adipose Tissue-Derived Stem Cells
Joint Authors
Shahar, Abraham
Gomes, Eduardo D.
Silva, Nuno A.
Oliveira, E.
Assunção-Silva, R. C.
Teixeira, F. G.
Ziv-Polat, Ofra
Salgado, Antonio
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-03
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Mesenchymal stem cells (MSCs) have been proposed for spinal cord injury (SCI) applications due to their capacity to secrete growth factors and vesicles—secretome—that impacts important phenomena in SCI regeneration.
To improve MSC survival into SCI sites, hydrogels have been used as transplantation vehicles.
Herein, we hypothesized if different hydrogels could interact differently with adipose tissue-derived MSCs (ASCs).
The efficacy of three natural hydrogels, gellan gum (functionalized with a fibronectin peptide), collagen, and a hydrogel rich in laminin epitopes (NVR-gel) in promoting neuritogenesis (alone and cocultured with ASCs), was evaluated in the present study.
Their impact on ASC survival, metabolic activity, and gene expression was also evaluated.
Our results indicated that all hydrogels supported ASC survival and viability, being this more evident for the functionalized GG hydrogels.
Moreover, the presence of different ECM-derived biological cues within the hydrogels appears to differently affect the mRNA levels of growth factors involved in neuronal survival, differentiation, and axonal outgrowth.
All the hydrogel-based systems supported axonal growth mediated by ASCs, but this effect was more robust in functionalized GG.
The data herein presented highlights the importance of biological cues within hydrogel-based biomaterials as possible modulators of ASC secretome and its effects for SCI applications.
American Psychological Association (APA)
Oliveira, E.& Assunção-Silva, R. C.& Ziv-Polat, Ofra& Gomes, Eduardo D.& Teixeira, F. G.& Silva, Nuno A.…[et al.]. 2017. Influence of Different ECM-Like Hydrogels on Neurite Outgrowth Induced by Adipose Tissue-Derived Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1201963
Modern Language Association (MLA)
Oliveira, E.…[et al.]. Influence of Different ECM-Like Hydrogels on Neurite Outgrowth Induced by Adipose Tissue-Derived Stem Cells. Stem Cells International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1201963
American Medical Association (AMA)
Oliveira, E.& Assunção-Silva, R. C.& Ziv-Polat, Ofra& Gomes, Eduardo D.& Teixeira, F. G.& Silva, Nuno A.…[et al.]. Influence of Different ECM-Like Hydrogels on Neurite Outgrowth Induced by Adipose Tissue-Derived Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1201963
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1201963