The Flavonoid Glabridin Induces OCT4 to Enhance Osteogenetic Potential in Mesenchymal Stem Cells
Joint Authors
Heo, June Seok
Lee, Seung Gwan
Kim, Hyun Ok
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-14
Country of Publication
Egypt
No. of Pages
10
Abstract EN
Mesenchymal stem cells (MSCs) are a promising tool for studying intractable diseases.
Unfortunately, MSCs can easily undergo cellular senescence during in vitro expansion by losing stemness.
The aim of this study was to improve the stemness and differentiation of MSCs by using glabridin, a natural flavonoid.
Assessments of cell viability, cell proliferation, β-galactosidase activity, differentiation, and gene expression by reverse transcription PCR were subsequently performed in the absence or presence of glabridin.
Glabridin enhanced the self-renewal capacity of MSCs, as indicated by the upregulation of the OCT4 gene.
In addition, it resulted in an increase in the osteogenic differentiation potential by inducing the expression of osteogenesis-related genes such as DLX5 and RUNX2.
We confirmed that glabridin improved the osteogenesis of MSCs with a significant elevation in the expression of OSTEOCALCIN and OSTEOPONTIN genes.
Taken together, these results suggest that glabridin enhances osteogenic differentiation of MSCs with induction of the OCT4 gene; thus, glabridin could be useful for stem cell-based therapies.
American Psychological Association (APA)
Heo, June Seok& Lee, Seung Gwan& Kim, Hyun Ok. 2017. The Flavonoid Glabridin Induces OCT4 to Enhance Osteogenetic Potential in Mesenchymal Stem Cells. Stem Cells International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1202001
Modern Language Association (MLA)
Heo, June Seok…[et al.]. The Flavonoid Glabridin Induces OCT4 to Enhance Osteogenetic Potential in Mesenchymal Stem Cells. Stem Cells International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1202001
American Medical Association (AMA)
Heo, June Seok& Lee, Seung Gwan& Kim, Hyun Ok. The Flavonoid Glabridin Induces OCT4 to Enhance Osteogenetic Potential in Mesenchymal Stem Cells. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1202001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202001