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Zinc-Induced SUMOylation of Dynamin-Related Protein 1 Protects the Heart against Ischemia-Reperfusion Injury
Joint Authors
Bian, Xiyun
Xu, Jingman
Zhao, Huanhuan
Zheng, Quan
Xiao, Xiaolin
Ma, Xiaofang
Li, Yanxia
Du, Xinping
Liu, Xiaozhi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Zinc plays a role in mitophagy and protects cardiomyocytes from ischemia/reperfusion injury.
This study is aimed at investigating whether SUMOylation of Drp1 is involved in the protection of zinc ion on cardiac I/R injury.
Methods.
Mouse hearts were subjected to 30 minutes of regional ischemia followed by 2 hours of reperfusion (ischemia/reoxygenation (I/R)).
Infarct size and apoptosis were assessed.
HL-1 cells were subjected to 24 hours of hypoxia and 6 hours of reoxygenation (hypoxia/reoxygenation (H/R)).
Zinc was given 5 min before reperfusion for 30 min.
SENP2 overexpression plasmid (Flag-SENP2), Drp1 mutation plasmid (Myc-Drp1 4KR), and SUMO1 siRNA were transfected into HL-1 cells for 48 h before hypoxia.
Effects of zinc on SUMO family members were analyzed by Western blotting.
SUMOylation of Drp1, apoptosis and the collapse of mitochondrial membrane potential (ΔΨm), and mitophagy were evaluated.
Results.
Compared with the control, SUMO1 modification level of proteins in the H/R decreased, while this effect was reversed by zinc.
In the setting of H/R, zinc attenuated myocardial apoptosis, which was reversed by SUMO1 siRNA.
Similar effects were observed in SUMO1 KO mice exposed to H/R.
In addition, the dynamin-related protein 1 (Drp1) is a target protein of SUMO1.
The SUMOylation of Drp1 induced by zinc regulated mitophagy and contributed to the protective effect of zinc on H/R injury.
Conclusions.
SUMOylation of Drp1 played an essential role in zinc-induced cardio protection against I/R injury.
Our findings provide a promising therapeutic approach for acute myocardial I/R injury.
American Psychological Association (APA)
Bian, Xiyun& Xu, Jingman& Zhao, Huanhuan& Zheng, Quan& Xiao, Xiaolin& Ma, Xiaofang…[et al.]. 2019. Zinc-Induced SUMOylation of Dynamin-Related Protein 1 Protects the Heart against Ischemia-Reperfusion Injury. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202045
Modern Language Association (MLA)
Bian, Xiyun…[et al.]. Zinc-Induced SUMOylation of Dynamin-Related Protein 1 Protects the Heart against Ischemia-Reperfusion Injury. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1202045
American Medical Association (AMA)
Bian, Xiyun& Xu, Jingman& Zhao, Huanhuan& Zheng, Quan& Xiao, Xiaolin& Ma, Xiaofang…[et al.]. Zinc-Induced SUMOylation of Dynamin-Related Protein 1 Protects the Heart against Ischemia-Reperfusion Injury. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202045
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202045