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L-Cystathionine Protects against Homocysteine-Induced Mitochondria-Dependent Apoptosis of Vascular Endothelial Cells
Joint Authors
Junbao, D.
Huang, Yaqian
Tang, Chaoshu
Jin, Hongfang
Wang, Yi
Wang, Xiuli
Zhang, Lulu
Zhang, Da
Bai, Lu
Kong, Wei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-25
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The study was aimed at investigating the effects of L-cystathionine on vascular endothelial cell apoptosis and its mechanisms.
Cultured human umbilical vein endothelial cells (HUVECs) were used in the study.
Apoptosis of vascular endothelial cells was induced by homocysteine.
Apoptosis, mitochondrial superoxide anion, mitochondrial membrane potential, mitochondrial permeability transition pore (MPTP) opening, and caspase-9 and caspase-3 activities were examined.
Expression of Bax, Bcl-2, and cleaved caspase-3 was tested and BTSA1, a Bax agonist, and HUVEC Bax overexpression was used in the study.
Results showed that homocysteine obviously induced the apoptosis of HUVECs, and this effect was significantly attenuated by the pretreatment with L-cystathionine.
Furthermore, L-cystathionine decreased the production of mitochondrial superoxide anion and the expression of Bax and restrained its translocation to mitochondria, increased mitochondrial membrane potential, inhibited mitochondrial permeability transition pore (MPTP) opening, suppressed the leakage of cytochrome c from mitochondria into the cytoplasm, and downregulated activities of caspase-9 and caspase-3.
However, BTSA1, a Bax agonist, or Bax overexpression successfully abolished the inhibitory effect of L-cystathionine on Hcy-induced MPTP opening, caspase-9 and caspase-3 activation, and HUVEC apoptosis.
Taken together, our results indicated that L-cystathionine could protect against homocysteine-induced mitochondria-dependent apoptosis of HUVECs.
American Psychological Association (APA)
Wang, Xiuli& Wang, Yi& Zhang, Lulu& Zhang, Da& Bai, Lu& Kong, Wei…[et al.]. 2019. L-Cystathionine Protects against Homocysteine-Induced Mitochondria-Dependent Apoptosis of Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202056
Modern Language Association (MLA)
Wang, Xiuli…[et al.]. L-Cystathionine Protects against Homocysteine-Induced Mitochondria-Dependent Apoptosis of Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1202056
American Medical Association (AMA)
Wang, Xiuli& Wang, Yi& Zhang, Lulu& Zhang, Da& Bai, Lu& Kong, Wei…[et al.]. L-Cystathionine Protects against Homocysteine-Induced Mitochondria-Dependent Apoptosis of Vascular Endothelial Cells. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202056
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202056