Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment
Joint Authors
Chimenti, Isotta
Angelini, Francesco
Messina, Elisa
Meglio, Franca Di
Castaldo, Clotilde
Nurzynska, Daria
Sciarretta, Sebastiano
Frati, G.
Biondi-Zoccai, Giuseppe
Pagano, Francesca
Picchio, Vittorio
Maiello, Ciro
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-27
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Human resident cardiac progenitor cells (CPCs) isolated as cardiosphere-derived cells (CDCs) are under clinical evaluation as a therapeutic product for cardiac regenerative medicine.
Unfortunately, limited engraftment and differentiation potential of transplanted cells significantly hamper therapeutic success.
Moreover, maladaptive remodelling of the extracellular matrix (ECM) during heart failure progression provides impaired biological and mechanical signals to cardiac cells, including CPCs.
In this study, we aimed at investigating the differential effect on the phenotype of human CDCs of cardiac fibroblast-derived ECM substrates from healthy or diseased hearts, named, respectively, normal or pathological cardiogel (CG-N/P).
After 7 days of culture, results show increased levels of cardiogenic gene expression (NKX2.5, CX43) on both decellularized cardiogels compared to control, while the proportion and staining patterns of GATA4, OCT4, NKX2.5, ACTA1, VIM, and CD90-positive CPCs were not affected, as assessed by immunofluorescence microscopy and flow cytometry analyses.
Nonetheless, CDCs cultured on CG-N secreted significantly higher levels of osteopontin, FGF6, FGF7, NT-3, IGFBP4, and TIMP-2 compared to those cultured on CG-P, suggesting overall a reduced trophic and antiremodelling paracrine profile of CDCs when in contact with ECM from pathological cardiac fibroblasts.
These results provide novel insights into the bidirectional interplay between cardiac ECM and CPCs, potentially affecting CPC biology and regenerative potential.
American Psychological Association (APA)
Pagano, Francesca& Angelini, Francesco& Castaldo, Clotilde& Picchio, Vittorio& Messina, Elisa& Sciarretta, Sebastiano…[et al.]. 2017. Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment. Stem Cells International،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202127
Modern Language Association (MLA)
Pagano, Francesca…[et al.]. Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment. Stem Cells International No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1202127
American Medical Association (AMA)
Pagano, Francesca& Angelini, Francesco& Castaldo, Clotilde& Picchio, Vittorio& Messina, Elisa& Sciarretta, Sebastiano…[et al.]. Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1202127
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202127