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Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma
Joint Authors
Garcia-Castro, Javier
Zarzosa, Patricia
Vidal, Isaac
Molist, Carla
Giralt, Irina
Navarro, Natalia
Soriano, Aroa
Segura, Miguel F.
Alfranca, Arantza
Sánchez de Toledo, José
Roma, Josep
Gallego, Soledad
Almazán-Moga, Anna
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-24
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Rhabdomyosarcoma (RMS) is the most common type of soft tissue sarcoma in children and can be divided into two main subtypes: embryonal (eRMS) and alveolar (aRMS).
Among the cellular heterogeneity of tumors, the existence of a small fraction of cells called cancer stem cells (CSC), thought to be responsible for the onset and propagation of cancer, has been demonstrated in some neoplasia.
Although the existence of CSC has been reported for eRMS, their existence in aRMS, the most malignant subtype, has not been demonstrated to date.
Given the lack of suitable markers to identify this subpopulation in aRMS, we used cancer stem cell-enriched supracellular structures (spheres and holoclones) to study this subpopulation.
This strategy allowed us to demonstrate the capacity of both aRMS and eRMS cells to form these structures and retain self-renewal capacity.
Furthermore, cells contained in spheres and holoclones showed significant Hedgehog pathway induction, the inhibition of which (pharmacologic or genetic) impairs the formation of both holoclones and spheres.
Our findings point to a crucial role of this pathway in the maintenance of these structures and suggest that Hedgehog pathway targeting in CSC may have great potential in preventing local relapses and metastases.
American Psychological Association (APA)
Almazán-Moga, Anna& Zarzosa, Patricia& Vidal, Isaac& Molist, Carla& Giralt, Irina& Navarro, Natalia…[et al.]. 2017. Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma. Stem Cells International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202146
Modern Language Association (MLA)
Almazán-Moga, Anna…[et al.]. Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma. Stem Cells International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1202146
American Medical Association (AMA)
Almazán-Moga, Anna& Zarzosa, Patricia& Vidal, Isaac& Molist, Carla& Giralt, Irina& Navarro, Natalia…[et al.]. Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202146
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202146