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Gamma Radiation-Induced Disruption of Cellular Junctions in HUVECs Is Mediated through Affecting MAPKNF-κB Inflammatory Pathways
Joint Authors
Tang, Feng Ru
Wang, H.
Segaran, R. Chandra
Chan, L. Y.
Aladresi, Aref A. M.
Chinnathambi, A.
Alharbi, S. A.
Sethi, Gautam
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-04
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Ionizing radiation-induced cardiovascular diseases (CVDs) have been well documented.
However, the mechanisms of CVD genesis are still not fully understood.
In this study, human umbilical vein endothelial cells (HUVECs) were exposed to gamma irradiation at different doses ranging from 0.2 Gy to 5 Gy.
Cell viability, migration ability, permeability, oxidative and nitrosative stresses, inflammation, and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) pathway activation were evaluated postirradiation.
It was found that gamma irradiation at doses ranging from 0.5 Gy to 5 Gy inhibited the migration ability of HUVECs without any significant effects on cell viability at 6 h and 24 h postirradiation.
The decreased transendothelial electrical resistance (TEER), increased permeability, and disruption of cellular junctions were observed in HUVECs after gamma irradiation accompanied by the lower levels of junction-related proteins such as ZO-1, occludin, vascular endothelial- (VE-) cadherin, and connexin 40.
The enhanced oxidative and nitrosative stresses, e.g., ROS and NO2- levels and inflammatory cytokines IL-6 and TNF-α were demonstrated in HUVECs after gamma irradiation.
Western blot results showed that protein levels of mitogen-activated protein kinase (MAPK) pathway molecules p38, p53, p21, and p27 increased after gamma irradiation, which further induced the activation of the NF-κB pathway.
BAY 11-7085, an inhibitor of NF-κB activation, was demonstrated to partially block the effects of gamma radiation in HUVECs examined by TEER and FITC-dextran permeability assay.
We therefore concluded that the gamma irradiation-induced disruption of cellular junctions in HUVECs was through the inflammatory MAPK/NF-κB signaling pathway.
American Psychological Association (APA)
Wang, H.& Segaran, R. Chandra& Chan, L. Y.& Aladresi, Aref A. M.& Chinnathambi, A.& Alharbi, S. A.…[et al.]. 2019. Gamma Radiation-Induced Disruption of Cellular Junctions in HUVECs Is Mediated through Affecting MAPKNF-κB Inflammatory Pathways. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202194
Modern Language Association (MLA)
Wang, H.…[et al.]. Gamma Radiation-Induced Disruption of Cellular Junctions in HUVECs Is Mediated through Affecting MAPKNF-κB Inflammatory Pathways. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1202194
American Medical Association (AMA)
Wang, H.& Segaran, R. Chandra& Chan, L. Y.& Aladresi, Aref A. M.& Chinnathambi, A.& Alharbi, S. A.…[et al.]. Gamma Radiation-Induced Disruption of Cellular Junctions in HUVECs Is Mediated through Affecting MAPKNF-κB Inflammatory Pathways. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202194