Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid
Joint Authors
Vorotelyak, Ekaterina
Kalabusheva, Ekaterina
Terskikh, Vasily
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-10
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Hair follicle (HF) reconstruction in vitro is a promising field in alopecia treatment and human HF development research.
Here, we combined postnatal human dermal papilla (DP) cells and skin epidermal keratinocytes (KCs) in a hanging drop culture to develop an artificial HF germ.
The method is based on DP cell hair-inducing properties and KC self-organization.
We evaluated two protocols of aggregate assembling.
Mixed HF germ-like structures demonstrated the initiation of epithelial-mesenchymal interaction, including WNT pathway activation and expression of follicular markers.
We analyzed the influence of possible DP cell niche components including soluble factors and extracellular matrix (ECM) molecules in the process of the organoid assembling and growth.
Our results demonstrated that soluble factors had little impact on HF germ generation and Ki67+ cell score inside the organoids although BMP6 and VD3 maintained effectively the DP identity in the monolayer culture.
Aggrecan, biglycan, fibronectin, and hyaluronic acid (HA) significantly stimulated cell proliferation in DP cell monolayer culture without any effect on DP cell identity.
Most of ECM compounds prevented the formation of cell aggregates while HA promoted the formation of larger organoids.
In conclusion, our model could be suitable to study cell-cell and cell-niche interactions during HF reconstruction in vitro.
American Psychological Association (APA)
Kalabusheva, Ekaterina& Terskikh, Vasily& Vorotelyak, Ekaterina. 2017. Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid. Stem Cells International،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202355
Modern Language Association (MLA)
Kalabusheva, Ekaterina…[et al.]. Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid. Stem Cells International No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1202355
American Medical Association (AMA)
Kalabusheva, Ekaterina& Terskikh, Vasily& Vorotelyak, Ekaterina. Hair Germ Model In Vitro via Human Postnatal Keratinocyte-Dermal Papilla Interactions: Impact of Hyaluronic Acid. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1202355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202355