Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes
Joint Authors
Khundzhua, D. A.
Trubetskoj, Oleg A.
Trubetskaya, Olga E.
Patsaeva, S. V.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Advanced fluorescence analysis within the wide range of excitation wavelengths from 230 to 510 nm accompanied with chromatography was used to study natural chromophoric dissolved organic matter (CDOM) from three freshwater Karelian lakes.
The influence of excitation wavelength (λex) on fluorescence quantum yield and emission maximum position was determined.
The CDOM fluorescence quantum yield has reached a minimum at λex∼270–280 nm and a maximum at λex∼340–360 nm.
It was monotonously decreasing after 370 nm towards longer excitation wavelengths.
Analytical reversed-phase high-performance liquid chromatography with multiwavelength fluorescence detector characterized distribution of fluorophores between hydrophilic/hydrophobic CDOM parts.
This technique revealed “hidden” protein-like fluorophores for some CDOM fractions, in spite of the absence of protein-like fluorescence in the initial CDOM samples.
The humic-like fluorescence was documented for all hydrophilic and hydrophobic CDOM chromatographic peaks, and its intensity was decreasing along with peaks’ hydrophobicity.
On contrary, the protein-like fluorescence was found only in the hydrophobic peaks, and its intensity was increasing along with peaks’ hydrophobicity.
This work provides new data on the CDOM optical properties consistent with the formation of supramolecular assemblies controlled by association of low-molecular size components.
In addition, these data are very useful for understanding the CDOM function in the environment.
American Psychological Association (APA)
Patsaeva, S. V.& Khundzhua, D. A.& Trubetskoj, Oleg A.& Trubetskaya, Olga E.. 2018. Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes. Journal of Spectroscopy،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1202462
Modern Language Association (MLA)
Patsaeva, S. V.…[et al.]. Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes. Journal of Spectroscopy No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1202462
American Medical Association (AMA)
Patsaeva, S. V.& Khundzhua, D. A.& Trubetskoj, Oleg A.& Trubetskaya, Olga E.. Excitation-Dependent Fluorescence Quantum Yield for Freshwater Chromophoric Dissolved Organic Matter from Northern Russian Lakes. Journal of Spectroscopy. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1202462
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202462