4-Hydroxycoumarin Derivative: N-(diphenylmethyl)-2-[(2-oxo-2H-chromen-4-yl)oxy]acetamide Interaction with Human Serum Albumin
Joint Authors
Chinnathambi, Shanmugavel
Karthikeyan, Subramani
Rajendiran, Mangaiyarkarsi
Udayakumar, Kanniyappan
Manoharan, Arunkumar
Kandasamy, Saravanan
Hanagata, Nobutaka
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-16
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In this study, the interaction between the coumarin derivative: N-(diphenylmethyl)-2-[(2-oxo-2H-chromen-4-yl)oxy]acetamide, biologically active drug, and human serum albumin (HSA) was investigated by using various optical spectroscopy techniques along with the computational technique.
The results of steady-state fluorescence spectroscopy show that the static quenching occurred while increasing the coumarin drug concentration into HSA.
Also, the binding constant (K) and thermodynamical parameters of enthalpy change (ΔH°), entropy change (ΔS°), and Gibbs free energy change (ΔG°) were calculated at different temperatures (293 K, 298 K, and 303 K).
The results are in good agreement with those of molecular docking studies, and also, the docking study was carried out to understand the hydrogen bonding and hydrophobic interaction between human serum albumin and coumarin derivative.
In addition to the docking, charge distribution analysis was done to understand the internal stability of coumarin derivative active sites of human serum albumin.
Further time-resolved emission spectroscopy (TRES) studies were carried out between free HSA and HSA-coumarin complex, and the result confirms the presence of the drug in the protein molecule without cytotoxicity.
American Psychological Association (APA)
Chinnathambi, Shanmugavel& Karthikeyan, Subramani& Rajendiran, Mangaiyarkarsi& Udayakumar, Kanniyappan& Manoharan, Arunkumar& Kandasamy, Saravanan…[et al.]. 2018. 4-Hydroxycoumarin Derivative: N-(diphenylmethyl)-2-[(2-oxo-2H-chromen-4-yl)oxy]acetamide Interaction with Human Serum Albumin. Journal of Spectroscopy،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1202479
Modern Language Association (MLA)
Chinnathambi, Shanmugavel…[et al.]. 4-Hydroxycoumarin Derivative: N-(diphenylmethyl)-2-[(2-oxo-2H-chromen-4-yl)oxy]acetamide Interaction with Human Serum Albumin. Journal of Spectroscopy No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1202479
American Medical Association (AMA)
Chinnathambi, Shanmugavel& Karthikeyan, Subramani& Rajendiran, Mangaiyarkarsi& Udayakumar, Kanniyappan& Manoharan, Arunkumar& Kandasamy, Saravanan…[et al.]. 4-Hydroxycoumarin Derivative: N-(diphenylmethyl)-2-[(2-oxo-2H-chromen-4-yl)oxy]acetamide Interaction with Human Serum Albumin. Journal of Spectroscopy. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1202479
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202479