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Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro
Joint Authors
Fleury-Cappellesso, Sandrine
Jorgensen, Christian
Mackiewicz, Zygmunt
Denkovskij, Jaroslav
Bagdonas, Edvardas
Kusleviciute, Ilona
Unguryte, Ausra
Porvaneckas, Narunas
Venalis, Algirdas
Bernotiene, Eiva
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-27
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Adipose tissue represents an abundant source of stem cells.
Along with anti-inflammatory effects, ASC secrete various factors that may modulate metabolism of extracellular matrix in osteoarthritic (OA) cartilage, suggesting that the presence of ASC could be advantageous for OA cartilage due to the recovery of homeostasis between matrix metalloproteinases (MMPs) and their tissue inhibitors of metalloproteinases (TIMPs).
To evaluate these effects, cartilage explants (CE) were cocultured with ASC for 3 and 7 days under stimulation with or without IL-1β.
The pattern of gene expression in CE was modified by ASC, including the upregulation of COL1A1 and COL3A1 and the downregulation of MMP13 and COL10A1.
The production of MMP-1, MMP-3, and MMP-13 by ASC was not significant; moreover, cocultures with ASC reduced MMP-13 production in CE.
In conclusion, active production of TIMP-1, TIMP-2, TIMP-3, IL-6, IL-8, and gelatinases MMP-2 and MMP-9 by ASC may be involved in the extracellular matrix remodelling, as indicated by the altered expression of collagens, the downregulated production of MMP-13, and the reduced chondrocyte apoptosis in the cocultured CE.
These data suggest that ASC modulated homeostasis of MMPs/TIMPs in degenerated OA cartilage in vitro and might be favourable in case of the intra-articular application of ASC therapy for the treatment of OA.
American Psychological Association (APA)
Denkovskij, Jaroslav& Bagdonas, Edvardas& Kusleviciute, Ilona& Mackiewicz, Zygmunt& Unguryte, Ausra& Porvaneckas, Narunas…[et al.]. 2017. Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro. Stem Cells International،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1202527
Modern Language Association (MLA)
Denkovskij, Jaroslav…[et al.]. Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro. Stem Cells International No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1202527
American Medical Association (AMA)
Denkovskij, Jaroslav& Bagdonas, Edvardas& Kusleviciute, Ilona& Mackiewicz, Zygmunt& Unguryte, Ausra& Porvaneckas, Narunas…[et al.]. Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro. Stem Cells International. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1202527
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202527