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Mitochondria-Targeted Peptide SS31 Attenuates Renal Tubulointerstitial Injury via Inhibiting Mitochondrial Fission in Diabetic Mice
Joint Authors
Yang, Shikun
Song, Panai
Li, Ai-mei
Han, Ya-chun
Peng, Can-hui
Song, Na
Yang, Ming
Zhan, Ming
Zeng, Ling-feng
Zhang, Wei
Tang, Shi-qi
Zhang, Hao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-02
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Objective.
Renal tubular injury is an early characteristic of diabetic nephropathy (DN) that is related to mitochondrial dysfunction.
In this study, we explore the effects and mechanisms of mitochondria-targeted peptide SS31 on renal tubulointerstitial injury in DN.
Method.
40 C57BL/6 mice were randomly divided into control group, STZ group, STZ+SS31 group, and STZ+normal saline group.
SS31 was intraperitoneally injected to the mice every other day for 24 weeks.
Renal lesions and the expression of Drp1, Mfn1, Bcl-2, Bax, Caspase1, IL-1β, and FN were detected.
In in vitro studies, HK-2 cells were incubated with different concentrations of D-glucose (5, 30 mM) or combined with SS31 and Drp1 inhibitor Midivi1.
Mitochondrial ROS, membrane potential, and morphology have been detected to evaluate the mitochondrial function.
Results.
Compared with diabetic mice, the levels of serum creatinine and microalbuminuria were significantly decreased in the SS31 group.
Renal tubulointerstitial fibrosis, oxidative stress, and apoptosis were observed in diabetic mice, while the pathological changes were reduced in the SS31-treatment group.
SS31 could decrease the expression of Drp1, Bax, Caspase1, IL-1β, and FN in the renal tissue of diabetic mice, while increasing the expression of Mfn1.
Additionally, mitochondria exhibit focal enlargement and crista swelling in renal tubular cells of diabetic mice, while SS31 treatment could partially block these changes.
An in vitro study showed that pretreatment with SS31 or Drp1 inhibitor Mdivi1 could restore the level of mitochondrial ROS, the membrane potential levels, and the expressions of Drp1, Bax, Caspase1, IL-1β, and FN in HK-2 cells under high-glucose conditions.
Conclusion.
SS31 protected renal tubulointerstitial injury in diabetic mice through a decrease in mitochondrial fragmentation via suppressing the expression of Drp1 and increasing the expression of Mfn1.
American Psychological Association (APA)
Yang, Shikun& Li, Ai-mei& Han, Ya-chun& Peng, Can-hui& Song, Na& Yang, Ming…[et al.]. 2019. Mitochondria-Targeted Peptide SS31 Attenuates Renal Tubulointerstitial Injury via Inhibiting Mitochondrial Fission in Diabetic Mice. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202608
Modern Language Association (MLA)
Yang, Shikun…[et al.]. Mitochondria-Targeted Peptide SS31 Attenuates Renal Tubulointerstitial Injury via Inhibiting Mitochondrial Fission in Diabetic Mice. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1202608
American Medical Association (AMA)
Yang, Shikun& Li, Ai-mei& Han, Ya-chun& Peng, Can-hui& Song, Na& Yang, Ming…[et al.]. Mitochondria-Targeted Peptide SS31 Attenuates Renal Tubulointerstitial Injury via Inhibiting Mitochondrial Fission in Diabetic Mice. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202608
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202608