Testicular Toxicity of Water Pipe Smoke Exposure in Mice and the Effect of Treatment with Nootkatone Thereon

Joint Authors

al-Salam, Suhail
Nemmar, Abderrahim
Yuvaraju, Priya
Beegam, Sumaya
Ali, Badreldin H.
Adham, Sirin A.
Al Balushi, Khalid
Al Za'abi, Mohammed
Manoj, Priyadarsini

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-06-25

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Biology

Abstract EN

There is a worldwide increase in the popularity of water pipe (shisha) tobacco smoking including in Europe and North America.

However, little is known about the effects of water pipe smoke (WPS) exposure on male reproductivity.

We have recently demonstrated that WPS exposure in mice induces testicular toxicity including inflammation and oxidative stress.

Nootkatone, a sesquiterpenoid found in grapefruit, has antioxidant and anti-inflammatory effects.

However, the possible protective effect of nootkatone on WPS-induced testicular toxicity has not been reported before.

Here, we tested the effects of treatment of mice with nootkatone on WPS-induced testicular toxicity.

Mice were exposed to normal air or WPS (30 minutes/day, for 30 days).

Nootkatone (90 mg/kg) was given orally to mice by gavage, 1 h before WPS or air exposure.

Nootkatone treatment significantly ameliorated the WPS-induced increase in plasma levels of inhibin, uric acid, and lactate dehydrogenase activity.

Nootkatone also significantly mitigated the decrease in testosterone, androgen-binding protein, and estradiol concentrations in the plasma induced by WPS.

In testicular homogenates, WPS exposure caused a decrease in the total nitric oxide level and an increase in the proinflammatory cytokine interleukin-1β level and oxidative stress markers including malondialdehyde, cytochrome C, and 8-Oxo-2′-deoxyguanosine.

All the latter effects were significantly alleviated by nootkatone treatment.

Moreover, in testicular homogenate, nootkatone inhibited the expression of nuclear factor-kappaB induced by WPS.

Likewise, histological examination of mouse testes showed that nootkatone treatment ameliorated the deterioration of spermatogenesis induced by WPS exposure.

We conclude that nootkatone ameliorated the WPS-induced testicular inflammation and oxidative stress and hormonal and spermatogenesis alterations.

American Psychological Association (APA)

Ali, Badreldin H.& al-Salam, Suhail& Adham, Sirin A.& Al Balushi, Khalid& Al Za'abi, Mohammed& Beegam, Sumaya…[et al.]. 2019. Testicular Toxicity of Water Pipe Smoke Exposure in Mice and the Effect of Treatment with Nootkatone Thereon. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202654

Modern Language Association (MLA)

Ali, Badreldin H.…[et al.]. Testicular Toxicity of Water Pipe Smoke Exposure in Mice and the Effect of Treatment with Nootkatone Thereon. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1202654

American Medical Association (AMA)

Ali, Badreldin H.& al-Salam, Suhail& Adham, Sirin A.& Al Balushi, Khalid& Al Za'abi, Mohammed& Beegam, Sumaya…[et al.]. Testicular Toxicity of Water Pipe Smoke Exposure in Mice and the Effect of Treatment with Nootkatone Thereon. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202654

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1202654