Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi
Joint Authors
Chandramani-Shivalingappa, Prashanth
Bhandari, Mahesh
Wiechert, Sarah A.
Gilbertie, Jessica
Jones, Douglas E.
Sponseller, Brett A.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Rhodococcus equi (R.
equi) is an intracellular macrophage-tropic pathogen with potential for causing fatal pyogranulomatous pneumonia in foals between 1 and 6 months of age.
In this study, we sought to determine whether infection of macrophages with R.
equi could lead to the induction of autophagy.
Murine bone marrow derived macrophages (BMDM) were infected with R.
equi for various time intervals and analyzed for upregulation of autophagy proteins and accumulation of autophagosomes relative to uninfected controls.
Western blot analysis showed a progressive increase in LC3-II and Beclin1 levels in a time-dependent manner.
The functional accumulation of autophagosomes detected with monodansylcadaverine further supported the enhanced induction of autophagy in BMDM infected with R.
equi.
In addition, infection of BMDM with R.
equi induced generation of reactive oxygen species (ROS) in a time-dependent manner.
These data are consistent with reports documenting the role of ROS in induction of autophagy and indicate that the infection of macrophages by R.
equi elicits innate host defense mechanisms.
American Psychological Association (APA)
Chandramani-Shivalingappa, Prashanth& Bhandari, Mahesh& Wiechert, Sarah A.& Gilbertie, Jessica& Jones, Douglas E.& Sponseller, Brett A.. 2017. Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi. Scientifica،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1202667
Modern Language Association (MLA)
Chandramani-Shivalingappa, Prashanth…[et al.]. Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi. Scientifica No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1202667
American Medical Association (AMA)
Chandramani-Shivalingappa, Prashanth& Bhandari, Mahesh& Wiechert, Sarah A.& Gilbertie, Jessica& Jones, Douglas E.& Sponseller, Brett A.. Induction of Reactive Intermediates and Autophagy-Related Proteins upon Infection of Macrophages with Rhodococcus equi. Scientifica. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1202667
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202667