Maternal Diet-Induced Obesity Compromises Oxidative Stress Status and Angiogenesis in the Porcine Placenta by Upregulating Nox2 Expression
Joint Authors
Tan, Chengquan
Yin, Yulong
Hu, Chengjun
Yang, Yunyu
Li, Jiaying
Wang, Hao
Cheng, Chuanhui
Yang, Linfang
Li, Qiqi
Deng, Jinping
Liang, Zuman
Xie, Zhengjun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-01
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Maternal obesity is associated with placental oxidative stress.
However, the mechanism underlying this association remains poorly understood.
In the present study, a gilt obesity model was developed by exposure to different energy diets and used to investigate the role of NADPH oxidase 2 (Nox2) in the placenta.
Specifically, 99 gilts (Guangdong Small-ear Spotted pig) at day 60 of gestation were randomly assigned to one of the following three treatments: low-energy group (L, DE=11.50 MJ/kg), medium-energy group (M, DE=12.41 MJ/kg), and high-energy group (H, DE=13.42 MJ/kg), with 11 replicate pens per treatment and 3 gilts per pen.
At the start of the study, maternal body weight and backfat thickness were not significantly different in the three treatments.
After the study, data indicated that the H group had higher body weight and backfat thickness gain for gilts during gestation and lower piglet birth weight compared with the other two groups.
Additionally, the H group showed glucolipid metabolic disorders and increased triglyceride and nonesterified fatty acid contents in the placenta of gilts.
Compared with the L group, the H group exhibited lower mitochondrial biogenesis and increased oxidative damage in the placenta.
Importantly, increased mRNA expression and protein abundance of Nox2 were observed for the first time in H group placentae.
Furthermore, compared with the L group, the H group showed a decrease in the density of placental vessels and the protein levels of vascular endothelial cadherin (VE-cadherin), vascular endothelial growth factor A (VEGF-A), and phosphorylation of vascular endothelial growth factor receptor 2 (p-VEGFR2) as well as the immunostaining intensity of platelet endothelial cell adhesion molecule-1 (CD31).
Our findings suggest that maternal high-energy diet-induced obesity increases placental oxidative stress and decreases placental angiogenesis possibly through the upregulation of Nox2.
American Psychological Association (APA)
Hu, Chengjun& Yang, Yunyu& Li, Jiaying& Wang, Hao& Cheng, Chuanhui& Yang, Linfang…[et al.]. 2019. Maternal Diet-Induced Obesity Compromises Oxidative Stress Status and Angiogenesis in the Porcine Placenta by Upregulating Nox2 Expression. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202705
Modern Language Association (MLA)
Hu, Chengjun…[et al.]. Maternal Diet-Induced Obesity Compromises Oxidative Stress Status and Angiogenesis in the Porcine Placenta by Upregulating Nox2 Expression. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1202705
American Medical Association (AMA)
Hu, Chengjun& Yang, Yunyu& Li, Jiaying& Wang, Hao& Cheng, Chuanhui& Yang, Linfang…[et al.]. Maternal Diet-Induced Obesity Compromises Oxidative Stress Status and Angiogenesis in the Porcine Placenta by Upregulating Nox2 Expression. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1202705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202705