Cerium Oxide Nanoparticles Regulate Insulin Sensitivity and Oxidative Markers in 3T3-L1 Adipocytes and C2C12 Myotubes
Joint Authors
González-Muniesa, Pedro
Lopez-Pascual, Amaya
Urrutia-Sarratea, Andoni
Lorente-Cebrián, Silvia
Martínez, J. Alfredo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Insulin resistance is associated with oxidative stress, mitochondrial dysfunction, and a chronic low-grade inflammatory status.
In this sense, cerium oxide nanoparticles (CeO2 NPs) are promising nanomaterials with antioxidant and anti-inflammatory properties.
Thus, we aimed to evaluate the effect of CeO2 NPs in mouse 3T3-L1 adipocytes, RAW 264.7 macrophages, and C2C12 myotubes under control or proinflammatory conditions.
Macrophages were treated with LPS, and both adipocytes and myotubes with conditioned medium (25% LPS-activated macrophages medium) to promote inflammation.
CeO2 NPs showed a mean size of ≤25.3 nm (96.7%) and a zeta potential of 30.57±0.58 mV, suitable for cell internalization.
CeO2 NPs reduced extracellular reactive oxygen species (ROS) in adipocytes with inflammation while increased in myotubes with control medium.
The CeO2 NPs increased mitochondrial content was observed in adipocytes under proinflammatory conditions.
Furthermore, the expression of Adipoq and Il10 increased in adipocytes treated with CeO2 NPs.
In myotubes, both Il1b and Adipoq were downregulated while Irs1 was upregulated.
Overall, our results suggest that CeO2 NPs could potentially have an insulin-sensitizing effect specifically on adipose tissue and skeletal muscle.
However, further research is needed to confirm these findings.
American Psychological Association (APA)
Lopez-Pascual, Amaya& Urrutia-Sarratea, Andoni& Lorente-Cebrián, Silvia& Martínez, J. Alfredo& González-Muniesa, Pedro. 2019. Cerium Oxide Nanoparticles Regulate Insulin Sensitivity and Oxidative Markers in 3T3-L1 Adipocytes and C2C12 Myotubes. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202841
Modern Language Association (MLA)
Lopez-Pascual, Amaya…[et al.]. Cerium Oxide Nanoparticles Regulate Insulin Sensitivity and Oxidative Markers in 3T3-L1 Adipocytes and C2C12 Myotubes. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1202841
American Medical Association (AMA)
Lopez-Pascual, Amaya& Urrutia-Sarratea, Andoni& Lorente-Cebrián, Silvia& Martínez, J. Alfredo& González-Muniesa, Pedro. Cerium Oxide Nanoparticles Regulate Insulin Sensitivity and Oxidative Markers in 3T3-L1 Adipocytes and C2C12 Myotubes. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1202841
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202841