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Luteolin Attenuates Cardiac IschemiaReperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function
Joint Authors
Xiao, Chi
Xia, Man-Li
Wang, Jue
Zhou, Xin-Ru
Lou, Yang-Yun
Tang, Li-Hui
Zhang, Feng-Jiang
Yang, Jin-Ting
Qian, Ling-Bo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Luteolin has been reported to attenuate ischemia/reperfusion (I/R) injury in the diabetic heart through endothelial nitric oxide synthase- (eNOS-) related antioxidative response.
Though the nuclear factor erythroid 2-related factor 2 (Nrf2) is regarded as a key endogenous factor to reduce diabetic oxidative stress, whether luteolin reduces cardiac I/R injury in the diabetic heart via enhancing Nrf2 function needs to be clarified.
We hypothesized that pretreatment with luteolin could alleviate cardiac I/R injury in the diabetic heart by affecting the eNOS/Nrf2 signaling pathway.
The diabetic rat was produced by a single injection of streptozotocin (65 mg/kg, i.p.) for 6 weeks, and then, luteolin (100 mg/kg/day, i.g.), eNOS inhibitor L-NAME, or Nrf2 inhibitor brusatol was administered for the succedent 2 weeks.
After that, the isolated rat heart was exposed to 30 min of global ischemia and 120 min of reperfusion to establish I/R injury.
Luteolin markedly ameliorated cardiac function and myocardial viability; upregulated expressions of heme oxygenase-1, superoxide dismutase, glutathione peroxidase, and catalase; and reduced myocardial lactate dehydrogenase release, malondialdehyde, and 8-hydroxydeoxyguanosine in the diabetic I/R heart.
All these ameliorating effects of luteolin were significantly reversed by L-NAME or brusatol.
Luteolin also markedly reduced S-nitrosylation of Kelch-like ECH-associated protein 1 (Keap1) and upregulated Nrf2 and its transcriptional activity.
This effect of luteolin on Keap1/Nrf2 signaling was attenuated by L-NAME.
These data reveal that luteolin protects the diabetic heart against I/R injury by enhancing eNOS-mediated S-nitrosylation of Keap1, with subsequent upregulation of Nrf2 and the Nrf2-related antioxidative signaling pathway.
American Psychological Association (APA)
Zhou, Xin-Ru& Lou, Yang-Yun& Tang, Li-Hui& Zhang, Feng-Jiang& Yang, Jin-Ting& Qian, Ling-Bo…[et al.]. 2019. Luteolin Attenuates Cardiac IschemiaReperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1202861
Modern Language Association (MLA)
Zhou, Xin-Ru…[et al.]. Luteolin Attenuates Cardiac IschemiaReperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1202861
American Medical Association (AMA)
Zhou, Xin-Ru& Lou, Yang-Yun& Tang, Li-Hui& Zhang, Feng-Jiang& Yang, Jin-Ting& Qian, Ling-Bo…[et al.]. Luteolin Attenuates Cardiac IschemiaReperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1202861
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202861