The Preventive Effects and the Mechanisms of Action of Navel Orange Peel Hydroethanolic Extract, Naringin, and Naringenin in N-Acetyl-p-aminophenol-Induced Liver Injury in Wistar Rats

Joint Authors

Ahmed, Osama M.
Al-Muzafar, Hessah Mohammed
Amin, Kamal Adel
Fahim, Hanaa I.
Ahmed, Heba Y.
Ahmed, Rasha R.
El-Nahass, El-Shaymaa
Abdelazeem, Walaa H.

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-19, 19 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-03-26

Country of Publication

Egypt

No. of Pages

19

Main Subjects

Biology

Abstract EN

N-Acetyl-p-aminophenol (APAP) or acetaminophen is the most common drug ingredient worldwide.

It is found in more than 600 different over-the-counter and prescription medicines.

Its long-term and overdose use is highly toxic and may result in liver injury.

Thus, this study was designed to assess the preventive effects and to suggest the mechanisms of action of the navel orange peel hydroethanolic extract, naringin, and naringenin in APAP-induced hepatotoxicity in male Wistar rats.

APAP was administered to male Wistar rats at a dose level of 0.5 g/kg body weight (b.w.) by oral gavage every other day for 4 weeks.

APAP-administered rats were treated with the navel orange peel hydroethanolic extract (50 mg/kg b.w.), naringin (20 mg/kg b.w.), and naringenin (20 mg/kg b.w.) by oral gavage every other day during the same period of APAP administration.

The treatments of APAP-administered rats with the peel extract, naringin, and naringenin produced a significant decrease in the elevated serum AST, ALT, ALP, LDH, and GGT activities as well as total bilirubin and TNF-α levels while they induced a significant increase in the lowered serum albumin and IL-4 levels.

The treatments also resulted in a significant decrease in the elevated liver lipid peroxidation and enhanced the liver GSH content and SOD, GST, and GPx activities as compared with APAP-administered control; the peel extract was the most potent in improving the liver LPO, GSH content, and GPx activity.

In addition, the three treatments significantly downregulated the elevated hepatic proapoptotic mediators p53, Bax, and caspase-3 and significantly upregulated the suppressed antiapoptotic protein, Bcl-2, in APAP-administered rats.

In association, the treatments markedly amended the APAP-induced liver histopathological deteriorations that include hepatocyte steatosis, cytoplasmic vacuolization, hydropic degeneration, and necrosis together with mononuclear leucocytic and fibroblastic inflammatory cells’ infiltration.

In conclusion, the navel orange peel hydroethanolic extract, naringin, and naringenin may exert their hepatopreventive effects in APAP-administered rats via enhancement of the antioxidant defense system and suppression of inflammation and apoptosis.

American Psychological Association (APA)

Ahmed, Osama M.& Fahim, Hanaa I.& Ahmed, Heba Y.& Al-Muzafar, Hessah Mohammed& Ahmed, Rasha R.& Amin, Kamal Adel…[et al.]. 2019. The Preventive Effects and the Mechanisms of Action of Navel Orange Peel Hydroethanolic Extract, Naringin, and Naringenin in N-Acetyl-p-aminophenol-Induced Liver Injury in Wistar Rats. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1202881

Modern Language Association (MLA)

Ahmed, Osama M.…[et al.]. The Preventive Effects and the Mechanisms of Action of Navel Orange Peel Hydroethanolic Extract, Naringin, and Naringenin in N-Acetyl-p-aminophenol-Induced Liver Injury in Wistar Rats. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-19.
https://search.emarefa.net/detail/BIM-1202881

American Medical Association (AMA)

Ahmed, Osama M.& Fahim, Hanaa I.& Ahmed, Heba Y.& Al-Muzafar, Hessah Mohammed& Ahmed, Rasha R.& Amin, Kamal Adel…[et al.]. The Preventive Effects and the Mechanisms of Action of Navel Orange Peel Hydroethanolic Extract, Naringin, and Naringenin in N-Acetyl-p-aminophenol-Induced Liver Injury in Wistar Rats. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-19.
https://search.emarefa.net/detail/BIM-1202881

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1202881