Cisplatin-Induced Stria Vascularis Damage Is Associated with Inflammation and Fibrosis
Joint Authors
Zhang, Na
Cai, Jing
Xu, Lei
Wang, Haibo
Liu, Wenwen
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell (HC) mechanotransduction and hearing.
Cell junctions are indispensable for the establishment of compositionally distinct fluid compartments in the inner ear.
Ototoxic drug cisplatin can damage SV and cause sensorineural hearing loss; however, the underlying mechanisms behind such injury are unclear.
In this study, after the intraperitoneal injection of cisplatin (3 mg/kg/day for 7 days) in mice, we determined the auditory function by EP recording and auditory brainstem response (ABR) analysis, observed the ultrastructure of SV by transmission electron microscopy (TEM), and examined the expression and distribution of cell junction proteins by western blot, PCR, and immunofluorescence staining.
We discovered that the EP was significantly reduced while ABR thresholds were significantly elevated in cisplatin-treated mice; cisplatin induced ultrastructural changes in marginal cells (MCs), endothelial cells (ECs), pericytes, etc.
We found that cisplatin insulted auditory function not only by reducing the expression of zonula occludens protein-1 (ZO-1) in MCs of the SV but also by decreasing the expression of connexin 26 (Cx26) and connexin 43 (Cx43) in MCs and basal cells (BCs).
More importantly, cisplatin induced activations of perivascular-resident macrophage-like melanocytes (PVM/Ms) and interleukin-1beta (IL-1β) as well as increased expressions of profibrotic proteins such as laminin and collagen IV in SV.
Thus, our results firstly showed that cisplatin induced fibrosis, inflammation, and the complex expression change of cell junctions in SV.
American Psychological Association (APA)
Zhang, Na& Cai, Jing& Xu, Lei& Wang, Haibo& Liu, Wenwen. 2020. Cisplatin-Induced Stria Vascularis Damage Is Associated with Inflammation and Fibrosis. Neural Plasticity،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1202944
Modern Language Association (MLA)
Zhang, Na…[et al.]. Cisplatin-Induced Stria Vascularis Damage Is Associated with Inflammation and Fibrosis. Neural Plasticity No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1202944
American Medical Association (AMA)
Zhang, Na& Cai, Jing& Xu, Lei& Wang, Haibo& Liu, Wenwen. Cisplatin-Induced Stria Vascularis Damage Is Associated with Inflammation and Fibrosis. Neural Plasticity. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1202944
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202944