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Transport of Reactive Oxygen and Nitrogen Species across Aquaporin: A Molecular Level Picture
Joint Authors
Yusupov, Maksudbek
Razzokov, Jamoliddin
Cordeiro, Rodrigo M.
Bogaerts, Annemie
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Aquaporins (AQPs) are transmembrane proteins that conduct not only water molecules across the cell membrane but also other solutes, such as reactive oxygen and nitrogen species (RONS), produced (among others) by cold atmospheric plasma (CAP).
These RONS may induce oxidative stress in the cell interior, which plays a role in cancer treatment.
The underlying mechanisms of the transport of RONS across AQPs, however, still remain obscure.
We apply molecular dynamics simulations to investigate the permeation of both hydrophilic (H2O2 and OH) and hydrophobic (NO2 and NO) RONS through AQP1.
Our simulations show that these RONS can all penetrate across the pores of AQP1.
The permeation free energy barrier of OH and NO is lower than that of H2O2 and NO2, indicating that these radicals may have easier access to the pore interior and interact with the amino acid residues of AQP1.
We also study the effect of RONS-induced oxidation of both the phospholipids and AQP1 (i.e., sulfenylation of Cys191) on the transport of the above-mentioned RONS across AQP1.
Both lipid and protein oxidation seem to slightly increase the free energy barrier for H2O2 and NO2 permeation, while for OH and NO, we do not observe a strong effect of oxidation.
The simulation results help to gain insight in the underlying mechanisms of the noticeable rise of CAP-induced RONS in cancer cells, thereby improving our understanding on the role of AQPs in the selective anticancer capacity of CAP.
American Psychological Association (APA)
Yusupov, Maksudbek& Razzokov, Jamoliddin& Cordeiro, Rodrigo M.& Bogaerts, Annemie. 2019. Transport of Reactive Oxygen and Nitrogen Species across Aquaporin: A Molecular Level Picture. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202998
Modern Language Association (MLA)
Yusupov, Maksudbek…[et al.]. Transport of Reactive Oxygen and Nitrogen Species across Aquaporin: A Molecular Level Picture. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1202998
American Medical Association (AMA)
Yusupov, Maksudbek& Razzokov, Jamoliddin& Cordeiro, Rodrigo M.& Bogaerts, Annemie. Transport of Reactive Oxygen and Nitrogen Species across Aquaporin: A Molecular Level Picture. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1202998
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1202998