Modulation of Antioxidant Potential with Coenzyme Q10 Suppressed Invasion of Temozolomide-Resistant Rat Glioma In Vitro and In Vivo
Joint Authors
Perez Garcia, Victor M.
Pešić, Milica
Burić, Sonja Stojković
Podolski-Renić, Ana
Dinić, Jelena
Stanković, Tijana
Jovanović, Mirna
Hadžić, Stefan
Ayuso, Jose M.
Virumbrales-Muñoz, María
Fernández, Luis J.
Ochoa, Ignacio
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-11
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The main reasons for the inefficiency of standard glioblastoma (GBM) therapy are the occurrence of chemoresistance and the invasion of GBM cells into surrounding brain tissues.
New therapeutic approaches obstructing these processes may provide substantial survival improvements.
The purpose of this study was to assess the potential of lipophilic antioxidant coenzyme Q10 (CoQ10) as a scavenger of reactive oxygen species (ROS) to increase sensitivity to temozolomide (TMZ) and suppress glioma cell invasion.
To that end, we used a previously established TMZ-resistant RC6 rat glioma cell line, characterized by increased production of ROS, altered antioxidative capacity, and high invasion potential.
CoQ10 in combination with TMZ exerted a synergistic antiproliferative effect.
These results were confirmed in a 3D model of microfluidic devices showing that the CoQ10 and TMZ combination is more cytotoxic to RC6 cells than TMZ monotherapy.
In addition, cotreatment with TMZ increased expression of mitochondrial antioxidant enzymes in RC6 cells.
The anti-invasive potential of the combined treatment was shown by gelatin degradation, Matrigel invasion, and 3D spheroid invasion assays as well as in animal models.
Inhibition of MMP9 gene expression as well as decreased N-cadherin and vimentin protein expression implied that CoQ10 can suppress invasiveness and the epithelial to mesenchymal transition in RC6 cells.
Therefore, our data provide evidences in favor of CoQ10 supplementation to standard GBM treatment due to its potential to inhibit GBM invasion through modulation of the antioxidant capacity.
American Psychological Association (APA)
Burić, Sonja Stojković& Podolski-Renić, Ana& Dinić, Jelena& Stanković, Tijana& Jovanović, Mirna& Hadžić, Stefan…[et al.]. 2019. Modulation of Antioxidant Potential with Coenzyme Q10 Suppressed Invasion of Temozolomide-Resistant Rat Glioma In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1203055
Modern Language Association (MLA)
Burić, Sonja Stojković…[et al.]. Modulation of Antioxidant Potential with Coenzyme Q10 Suppressed Invasion of Temozolomide-Resistant Rat Glioma In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1203055
American Medical Association (AMA)
Burić, Sonja Stojković& Podolski-Renić, Ana& Dinić, Jelena& Stanković, Tijana& Jovanović, Mirna& Hadžić, Stefan…[et al.]. Modulation of Antioxidant Potential with Coenzyme Q10 Suppressed Invasion of Temozolomide-Resistant Rat Glioma In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1203055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203055