The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats
Joint Authors
Li, Huawei
Li, Wen
Jiang, Yi
Ni, Yusu
Chen, Diyan
Qiu, Danhong
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
The precise mechanisms of nerve regeneration remain unclear.
The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied.
Methods.
Adult male Wistar rats were used to model either (i) facial nerve injury (axotomy) or (ii) reinjury (chronic axotomy followed by a second axotomy within 5 months).
The rats were housed in the animal facility of the Eye and ENT Hospital of Shanghai Medical School, Fudan University (Shanghai, China).
Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry.
The number of surviving motoneurons was quantified, and facial nerve regeneration was examined using transmission electron microscopy.
Results.
Reinjury of the facial nerve 12 weeks after the first axotomy resulted in upregulation of GAP43 mRNA and protein expression in neurons ipsilateral to the axotomy; immunohistochemistry revealed that Shh expression was higher compared with control side facial nuclei at the same time point.
GAP43 expression subsequently decreased.
Conclusion.
The greatest regeneration potential of the facial nerve occurred within 5 months following chronic axotomy in rats, and regeneration may involve the Shh signaling pathway.
American Psychological Association (APA)
Ni, Yusu& Chen, Diyan& Jiang, Yi& Qiu, Danhong& Li, Wen& Li, Huawei. 2020. The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats. Neural Plasticity،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1203106
Modern Language Association (MLA)
Ni, Yusu…[et al.]. The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats. Neural Plasticity No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1203106
American Medical Association (AMA)
Ni, Yusu& Chen, Diyan& Jiang, Yi& Qiu, Danhong& Li, Wen& Li, Huawei. The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats. Neural Plasticity. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1203106
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203106