Aβ-Induced Repressor Element 1-Silencing Transcription Factor (REST)‎ Gene Delivery Suppresses Activation of Microglia-Like BV-2 Cells

Abstract EN

Compelling evidence from basic molecular biology has demonstrated the crucial role of microglia in the pathogenesis of Alzheimer’s disease (AD).

Microglia were believed to play a dual role in both promoting and inhibiting Alzheimer’s disease progression.

It is of great significance to regulate the function of microglia and make them develop in a favorable way.

In the present study, we investigated the function of repressor element 1-silencing transcription factor (REST) in Aβ1-42-induced BV-2 cell dysfunction.

We concluded that Aβ1-42 could promote type I activation of BV-2 cells and induce cell proliferation, migration, and proinflammation cytokine TNF-α, IL-1β, and IL-6 expression.

Meanwhile, REST was upregulated, and nuclear translocalization took place due to Aβ1-42 stimulation.

When REST was knocked down by a specific short hairpin RNA (sh-RNA), BV-2 cell proliferation, migration, and proinflammation cytokine expression and secretion induced by Aβ1-42 were increased, demonstrating that REST may act as a repressor of microglia-like BV-2 cell activation.