Alternative Splicing of Cdh23 Exon 68 Is Regulated by RBM24, RBM38, and PTBP1
Joint Authors
Ren, Rui
Li, Nana
Wang, Yanfei
Du, Haibo
Xu, Zhigang
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Alternative splicing plays a pivotal role in modulating the function of eukaryotic proteins.
In the inner ear, many genes undergo alternative splicing, and errors in this process lead to hearing loss.
Cadherin 23 (CDH23) forms part of the so-called tip links, which are indispensable for mechanoelectrical transduction (MET) in the hair cells.
Cdh23 gene contains 69 exons, and exon 68 is subjected to alternative splicing.
Exon 68 of the Cdh23 gene is spliced into its mRNA only in a few cell types including hair cells.
The mechanism responsible for the alternative splicing of Cdh23 exon 68 remains elusive.
In the present work, we performed a cell-based screening to look for splicing factors that regulate the splicing of Cdh23 exon 68.
RBM24 and RBM38 were identified to enhance the inclusion of Cdh23 exon 68.
The splicing of Cdh23 exon 68 is affected in Rbm24 knockdown or knockout cells.
Moreover, we also found that PTBP1 inhibits the inclusion of Cdh23 exon 68.
Taken together, we show here that alternative splicing of Cdh23 exon 68 is regulated by RBM24, RBM38, and PTBP1.
American Psychological Association (APA)
Li, Nana& Du, Haibo& Ren, Rui& Wang, Yanfei& Xu, Zhigang. 2020. Alternative Splicing of Cdh23 Exon 68 Is Regulated by RBM24, RBM38, and PTBP1. Neural Plasticity،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1203157
Modern Language Association (MLA)
Li, Nana…[et al.]. Alternative Splicing of Cdh23 Exon 68 Is Regulated by RBM24, RBM38, and PTBP1. Neural Plasticity No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1203157
American Medical Association (AMA)
Li, Nana& Du, Haibo& Ren, Rui& Wang, Yanfei& Xu, Zhigang. Alternative Splicing of Cdh23 Exon 68 Is Regulated by RBM24, RBM38, and PTBP1. Neural Plasticity. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1203157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203157