Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats
Joint Authors
Ye, Yuqin
Yang, Yongxiang
Bai, Wei
Huang, Tao
Hei, Yue
Kang, Enming
Chen, Huijun
Kong, Chuiguang
He, Xiaosheng
Zhang, Xin
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
It was not clear how and whether neural stem cells (NSCs) responded to toll-like receptor 2 (TLR2) in the inflammatory environment after traumatic brain injury (TBI).
The current study investigated the correlation of TLR2 and NSC proliferation in the dentate gyrus (DG) using the TBI model of rats.
Immunofluorescence (IF) was used to observe the expression of BrdU, nestin, and TLR2 in the DG in morphology.
Proliferating cells in the DG were labelled by thymidine analog 5-bromo-2-deoxyuridine (BrdU).
Three-labelled BrdU, nestin, and DAPI was used for the identification of newly generated NSCs.
Western blotting and real-time polymerase chain reaction (PCR) were used to observe the expression of TLR2 from the level of protein and mRNA.
We observed that BrdU+/nestin+/DAPI+ cells accounted for 84.30%±6.54% among BrdU+ cells; BrdU+ and nestin+ cells in the DG were also TLR2+ cells.
BrdU+ cells and the expression of TLR2 (both protein and mRNA levels) both elevated immediately at 6 hours (h), 24 h, 3 days (d), and 7 d posttrauma and peaked in 3 d.
Results indicated that TLR2 was expressed on proliferating cells in the DG (NSCs possibly) and there was a potential correlation between increased TLR2 and proliferated NSCs after TBI.
Taken together, these findings suggested that TLR2 was involved in endogenous neurogenesis in the DG after TBI.
American Psychological Association (APA)
Zhang, Xin& Hei, Yue& Bai, Wei& Huang, Tao& Kang, Enming& Chen, Huijun…[et al.]. 2020. Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats. Neural Plasticity،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1203201
Modern Language Association (MLA)
Zhang, Xin…[et al.]. Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats. Neural Plasticity No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1203201
American Medical Association (AMA)
Zhang, Xin& Hei, Yue& Bai, Wei& Huang, Tao& Kang, Enming& Chen, Huijun…[et al.]. Toll-Like Receptor 2 Attenuates Traumatic Brain Injury-Induced Neural Stem Cell Proliferation in Dentate Gyrus of Rats. Neural Plasticity. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1203201
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203201