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Protection against HEMA-Induced Mitochondrial Injury In Vitro by Nrf2 Activation
Joint Authors
Jiao, Yang
Tay, Franklin R.
Niu, Tao
Liu, Huan
Chen, Ji-hua
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-07
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Dental resin monomers such as 2-hydroxyethyl methacrylate (HEMA) disturb vital cell functions and induce mitochondrial intrinsic apoptosis via generation of oxidative stress.
Nuclear factor erythroid 2-related factor 2 (Nrf2) regulates the gene expression of antioxidative enzymes and plays a crucial role in the maintenance of cellular redox equilibrium and mitochondrial homeostasis.
The present study investigated the functional significance of Nrf2 in cellular response toward HEMA.
It was found that HEMA stimulation promoted nuclear translocation of Nrf2 and increased Nrf2 and heme oxygenase-1 (HO-1) expression, which was further enhanced by Nrf2 activator tert-butylhydroquinone (tBHQ), but suppressed by Nrf2 inhibitor ML385.
Pretreatment of primary human dental pulp cells (hDPCs) with tBHQ protected the cells from HEMA-induced oxidative injury (increased reactive oxygen species production and apoptosis) and mitochondrial impairment (morphological alterations, decreased ATP production, suppressed oxidative phosphorylation activity, depolarization of mitochondrial membrane potential, and disrupted electron transport chain).
In contrast, pretreatment with ML385 increased cell sensitivity to these injurious processes.
This protective effect on mitochondria could be related to peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α)/nuclear respiratory factor 1 (NRF1) pathway.
These results contribute to the understanding of the function of Nrf2 and the development of novel therapies to counteract the adverse effects of dental resin monomers.
American Psychological Association (APA)
Jiao, Yang& Niu, Tao& Liu, Huan& Tay, Franklin R.& Chen, Ji-hua. 2019. Protection against HEMA-Induced Mitochondrial Injury In Vitro by Nrf2 Activation. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1203245
Modern Language Association (MLA)
Jiao, Yang…[et al.]. Protection against HEMA-Induced Mitochondrial Injury In Vitro by Nrf2 Activation. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1203245
American Medical Association (AMA)
Jiao, Yang& Niu, Tao& Liu, Huan& Tay, Franklin R.& Chen, Ji-hua. Protection against HEMA-Induced Mitochondrial Injury In Vitro by Nrf2 Activation. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1203245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203245