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Exosomes Mediate Hippocampal and Cortical Neuronal Injury Induced by Hepatic Ischemia-Reperfusion Injury through Activating Pyroptosis in Rats
Joint Authors
Zhang, Limei
Liu, Hanyu
Jia, Lili
Lyu, Jingshu
Sun, Ying
Yu, Hongli
Li, Hongxia
Liu, Weihua
Weng, Yiqi
Yu, Wenli
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-13
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Background.
The neuronal injury and cognitive dysfunction after liver transplantation have severe effects on the prognosis and life quality of patients.
Accumulating evidence suggests that both exosomes and pyroptosis could participate in hepatic ischemia-reperfusion injury (HIRI) and play key roles in neuronal death.
However, the link between exosomes and neuronal pyroptosis in HIRI awaits further investigation.
Methods.
After establishing the HIRI rat models, we primarily studied the role of pyroptosis in hippocampal and cortical neuron injury through detecting NOD-like receptor protein 3 (NLRP3), pro-caspase-1, cleaved-caspase-1, apoptosis-associated speck-like protein containing CARD (ASC), gasdermin D (GSDMD), interleukin-1beta (IL-1β), and interleukin-18 (IL-18) expressions with western blotting, immunohistochemical staining, and enzyme-linked immunosorbent assay (ELISA).
Then, we intravenously injected normal male rats with exosomes isolated from the sera of HIRI-challenged rats and pretreated rats with MCC950, a specific inhibitor of NLRP3, and carried out the same assay.
We also detected the levels of reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) in the hippocampal and cortical tissues.
Results.
The results indicated that NLRP3 inflammasome and caspase-1-dependent pyroptosis were activated in the hippocampus and cortex of HIRI rats.
Furthermore, serum-derived exosomes from HIRI-challenged rats not only had the ability to cross the blood-brain barrier (BBB) but also had the similar effects on neuronal pyroptosis.
Moreover, ROS and MDA productions were induced in the HIRI and exosome-challenged groups.
In addition, to some degree, MCC950 could alleviate HIRI-mediated hippocampal and cortical neuronal pyroptosis.
Conclusion.
This study experimentally demonstrated that circulating exosomes play a critical role in HIRI-mediated hippocampal and cortical injury through regulating neuronal pyroptosis.
American Psychological Association (APA)
Zhang, Limei& Liu, Hanyu& Jia, Lili& Lyu, Jingshu& Sun, Ying& Yu, Hongli…[et al.]. 2019. Exosomes Mediate Hippocampal and Cortical Neuronal Injury Induced by Hepatic Ischemia-Reperfusion Injury through Activating Pyroptosis in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1203383
Modern Language Association (MLA)
Zhang, Limei…[et al.]. Exosomes Mediate Hippocampal and Cortical Neuronal Injury Induced by Hepatic Ischemia-Reperfusion Injury through Activating Pyroptosis in Rats. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-1203383
American Medical Association (AMA)
Zhang, Limei& Liu, Hanyu& Jia, Lili& Lyu, Jingshu& Sun, Ying& Yu, Hongli…[et al.]. Exosomes Mediate Hippocampal and Cortical Neuronal Injury Induced by Hepatic Ischemia-Reperfusion Injury through Activating Pyroptosis in Rats. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-1203383
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203383