Porphyromonas gingivalis Induces Apoptosis and Autophagy via ER Stress in Human Umbilical Vein Endothelial Cells
Joint Authors
Hirasawa, Masaaki
Kurita-Ochiai, Tomoko
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
It has been reported that periodontitis is associated with an increased risk of atherosclerosis.
Accumulating evidence suggests that endothelial dysfunction is an early marker for atherosclerosis.
To determine how periodontal infections contribute to endothelial dysfunction, we examined the effect of Porphyromonas gingivalis on human umbilical vein endothelial cells (HUVEC).
P.
gingivalis significantly suppressed the viability of HUVEC, induced DNA fragmentation and annexin V staining, and increased caspase-3, caspase-8, and caspase-9 activities.
P.
gingivalis also increased the expression of GADD153 and GRP78 and caspase-12 activity.
Further, P.
gingivalis induced autophagy, as evidenced by increased LC3-II and Beclin-1 levels.
The suppression of P.
gingivalis-induced autophagy by silencing of LC3 with siRNA significantly increased P.
gingivalis-induced apoptosis.
ER stress inhibitor, salubrinal, suppressed apoptosis and autophagy by inhibiting P.
gingivalis-induced DNA fragmentation and LC3-II expression.
These data suggest that P.
gingivalis infection induces ER stress-mediated apoptosis followed by autophagic response that protects HUVEC from P.
gingivalis-mediated apoptosis, potentially amplifying proatherogenic mechanisms in the perturbed vasculature.
American Psychological Association (APA)
Hirasawa, Masaaki& Kurita-Ochiai, Tomoko. 2018. Porphyromonas gingivalis Induces Apoptosis and Autophagy via ER Stress in Human Umbilical Vein Endothelial Cells. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1203404
Modern Language Association (MLA)
Hirasawa, Masaaki& Kurita-Ochiai, Tomoko. Porphyromonas gingivalis Induces Apoptosis and Autophagy via ER Stress in Human Umbilical Vein Endothelial Cells. Mediators of Inflammation No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1203404
American Medical Association (AMA)
Hirasawa, Masaaki& Kurita-Ochiai, Tomoko. Porphyromonas gingivalis Induces Apoptosis and Autophagy via ER Stress in Human Umbilical Vein Endothelial Cells. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1203404
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203404