LncRNA LEGLTBC Functions as a ceRNA to Antagonize the Effects of miR-34a on the Downregulation of SIRT1 in Glucolipotoxicity-Induced INS-1 Beta Cell Oxidative Stress and Apoptosis
Joint Authors
Kong, Xiang
Zhang, Hongmei
Su, Qing
Lv, Kun
Yao, Xin-Ming
Hua, Qiang
Li, Xiao-Yong
Liu, Chong-xiao
Wang, Guo-dong
Yang, Hui
Ma, Ming-zhe
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-14
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Type 2 diabetes mellitus is a chronic metabolic disorder characterized by elevated blood glucose and/or high serum free fatty acids.
Chronic hyperlipidemia causes the dysfunction of pancreatic beta cells, which is aggravated in the presence of hyperglycemia (glucolipotoxicity).
Long noncoding RNAs (lncRNAs) have been suggested to play key roles in type 1 diabetes mellitus development.
However, their roles in glucolipotoxicity-induced beta cell dysfunction are not fully understood.
In the present study, we identified the differentially expressed lncRNAs in INS-1 cells exposed to high glucose and palmitate (HG/PA).
Among the dysregulated lncRNAs, NONRATT003679.2 (low expression in glucolipotoxicity-treated beta cells (LEGLTBC)) was involved in glucolipotoxicity-evoked rat islet beta cell damage.
LEGLTBC functioned as a molecular sponge of miR-34a in INS-1 cells.
Additionally, SIRT1 was identified as a target of miR-34a and LEGLTBC promoted SIRT1 expression by sponging miR-34a.
The upregulation of LEGLTBC attenuated HG/PA-induced INS-1 cell injury through the promotion of SIRT1-mediated suppression of ROS accumulation and apoptosis.
This is the first study to comprehensively identify the lncRNA expression profiling of HG/PA-treated INS-1 beta cells and to demonstrate that LEGLTBC functions as a competing endogenous RNA and regulates miR-34a/SIRT1-mediated oxidative stress and apoptosis in INS-1 cells undergoing glucolipotoxicity.
American Psychological Association (APA)
Kong, Xiang& Liu, Chong-xiao& Wang, Guo-dong& Yang, Hui& Yao, Xin-Ming& Hua, Qiang…[et al.]. 2019. LncRNA LEGLTBC Functions as a ceRNA to Antagonize the Effects of miR-34a on the Downregulation of SIRT1 in Glucolipotoxicity-Induced INS-1 Beta Cell Oxidative Stress and Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1203461
Modern Language Association (MLA)
Kong, Xiang…[et al.]. LncRNA LEGLTBC Functions as a ceRNA to Antagonize the Effects of miR-34a on the Downregulation of SIRT1 in Glucolipotoxicity-Induced INS-1 Beta Cell Oxidative Stress and Apoptosis. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1203461
American Medical Association (AMA)
Kong, Xiang& Liu, Chong-xiao& Wang, Guo-dong& Yang, Hui& Yao, Xin-Ming& Hua, Qiang…[et al.]. LncRNA LEGLTBC Functions as a ceRNA to Antagonize the Effects of miR-34a on the Downregulation of SIRT1 in Glucolipotoxicity-Induced INS-1 Beta Cell Oxidative Stress and Apoptosis. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1203461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203461