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Bisdemethoxycurcumin and Its Cyclized Pyrazole Analogue Differentially Disrupt Lipopolysaccharide Signalling in Human Monocyte-Derived Macrophages
Joint Authors
Fadini, Gian Paolo
Tedesco, Serena
Zusso, Morena
Facci, Laura
Trenti, Annalisa
Boscaro, Carlotta
Belluti, Federica
Bolego, Chiara
Cignarella, Andrea
Giusti, Pietro
Skaper, Stephen D.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Several studies suggest that curcumin and related compounds possess antioxidant and anti-inflammatory properties including modulation of lipopolysaccharide- (LPS-) mediated signalling in macrophage cell models.
We here investigated the effects of curcumin and the two structurally unrelated analogues GG6 and GG9 in primary human blood-derived macrophages as well as the signalling pathways involved.
Macrophages differentiated from peripheral blood monocytes for 7 days were activated with LPS or selective Toll-like receptor agonists for 24 h.
The effects of test compounds on cytokine production and immunophenotypes evaluated as CD80+/CCR2+ and CD206+/CD163+ subsets were examined by ELISA and flow cytometry.
Signalling pathways were probed by Western blot.
Curcumin (2.5–10 μM) failed to suppress LPS-induced inflammatory responses.
While GG6 reduced LPS-induced IκB-α degradation and showed a trend towards reduced interleukin-1β release, GG9 prevented the increase in proinflammatory CD80+ macrophage subset, downregulation of the anti-inflammatory CD206+/CD163+ subset, increase in p38 phosphorylation, and increase in cell-bound and secreted interleukin-1β stimulated by LPS, at least in part through signalling pathways not involving Toll-like receptor 4 and nuclear factor-κB.
Thus, the curcumin analogue GG9 attenuated the LPS-induced inflammatory response in human blood-derived macrophages and may therefore represent an attractive chemical template for macrophage pharmacological targeting.
American Psychological Association (APA)
Tedesco, Serena& Zusso, Morena& Facci, Laura& Trenti, Annalisa& Boscaro, Carlotta& Belluti, Federica…[et al.]. 2018. Bisdemethoxycurcumin and Its Cyclized Pyrazole Analogue Differentially Disrupt Lipopolysaccharide Signalling in Human Monocyte-Derived Macrophages. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1203490
Modern Language Association (MLA)
Tedesco, Serena…[et al.]. Bisdemethoxycurcumin and Its Cyclized Pyrazole Analogue Differentially Disrupt Lipopolysaccharide Signalling in Human Monocyte-Derived Macrophages. Mediators of Inflammation No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1203490
American Medical Association (AMA)
Tedesco, Serena& Zusso, Morena& Facci, Laura& Trenti, Annalisa& Boscaro, Carlotta& Belluti, Federica…[et al.]. Bisdemethoxycurcumin and Its Cyclized Pyrazole Analogue Differentially Disrupt Lipopolysaccharide Signalling in Human Monocyte-Derived Macrophages. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1203490
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203490