Green Tea Prevents NAFLD by Modulation of miR-34a and miR-194 Expression in a High-Fat Diet Mouse Model
Joint Authors
Cogliati, Bruno
Torres, L. F.
Otton, R.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-04
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Background/Aims.
Nonalcoholic fatty liver disease (NAFLD) is considered the hepatic manifestation of metabolic syndrome.
It is currently the most common chronic liver disease with complex pathogenesis and challenging treatment.
Here, we investigated the hepatoprotective role of green tea (GT) and determined the involvement of miRNAs and its mechanism of action.
Methods.
Male C57Bl/6 mice were fed with a high-fat diet for 4 weeks.
After this period, the animals received gavage with GT (500 mg/kg body weight) over 12 weeks (5 days/week).
HepG2 cell lines were transfected with miR-34a or miR-194 mimetics and inhibitors to validate the in vivo results or were treated with TNF-α to evaluate miRNA regulation.
Results.
GT supplementation protects against NAFLD development by altering lipid metabolism, increasing gene expression involved in triglycerides and fatty acid catabolism, and decreasing uptake and lipid accumulation.
This phenotype was accompanied by miR-34a downregulation and an increase in their mRNA targets Sirt1, Pparα, and Insig2.
GT upregulated hepatic miR-194 by inhibiting TNF-α action leading to a decrease in miR-194 target genes Hmgcs/Apoa5.
Conclusion.
Our study identified for the first time that the beneficial effects of GT in the liver can be due to the modulation of miRNAs, opening new perspectives for the treatment of NAFLD focusing on epigenetic regulation of miR-34a and miR-194 as green tea targets.
American Psychological Association (APA)
Torres, L. F.& Cogliati, Bruno& Otton, R.. 2019. Green Tea Prevents NAFLD by Modulation of miR-34a and miR-194 Expression in a High-Fat Diet Mouse Model. Oxidative Medicine and Cellular Longevity،Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1203515
Modern Language Association (MLA)
Torres, L. F.…[et al.]. Green Tea Prevents NAFLD by Modulation of miR-34a and miR-194 Expression in a High-Fat Diet Mouse Model. Oxidative Medicine and Cellular Longevity No. 2019 (2019), pp.1-18.
https://search.emarefa.net/detail/BIM-1203515
American Medical Association (AMA)
Torres, L. F.& Cogliati, Bruno& Otton, R.. Green Tea Prevents NAFLD by Modulation of miR-34a and miR-194 Expression in a High-Fat Diet Mouse Model. Oxidative Medicine and Cellular Longevity. 2019. Vol. 2019, no. 2019, pp.1-18.
https://search.emarefa.net/detail/BIM-1203515
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203515