Elevated Expression of AXL May Contribute to the Epithelial-to-Mesenchymal Transition in Inflammatory Bowel Disease Patients
Joint Authors
Varga, Csaba
Nagy, István
Kellermayer, Zoltán
Balogh, Péter
Boros, Éva
Strifler, Gerda
Vörös, Andrea
Sarlós, Patrícia
Vincze, Áron
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Understanding the molecular mechanisms inducing and regulating epithelial-to-mesenchymal transition (EMT) upon chronic intestinal inflammation is critical for understanding the exact pathomechanism of inflammatory bowel disease (IBD).
The aim of this study was to determine the expression profile of TAM family receptors in an inflamed colon.
For this, we used a rat model of experimental colitis and also collected samples from colons of IBD patients.
Samples were taken from both inflamed and uninflamed regions of the same colon; the total RNA was isolated, and the mRNA and microRNA expressions were monitored.
We have determined that AXL is highly induced in active-inflamed colon, which is accompanied with reduced expression of AXL-regulating microRNAs.
In addition, the expression of genes responsible for inducing or maintaining mesenchymal phenotype, such as SNAI1, ZEB2, VIM, MMP9, and HIF1α, were all significantly induced in the active-inflamed colon of IBD patients while the epithelial marker E-cadherin (CDH1) was downregulated.
We also show that, in vitro, monocytic and colonic epithelial cells increase the expression of AXL in response to LPS or TNFα stimuli, respectively.
In summary, we identified several interacting genes and microRNAs with mutually exclusive expression pattern in active-inflamed colon of IBD patients.
Our results shed light onto a possible AXL- and microRNA-mediated regulation influencing epithelial-to-mesenchymal transition in IBD.
American Psychological Association (APA)
Boros, Éva& Kellermayer, Zoltán& Balogh, Péter& Strifler, Gerda& Vörös, Andrea& Sarlós, Patrícia…[et al.]. 2018. Elevated Expression of AXL May Contribute to the Epithelial-to-Mesenchymal Transition in Inflammatory Bowel Disease Patients. Mediators of Inflammation،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1203539
Modern Language Association (MLA)
Boros, Éva…[et al.]. Elevated Expression of AXL May Contribute to the Epithelial-to-Mesenchymal Transition in Inflammatory Bowel Disease Patients. Mediators of Inflammation No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1203539
American Medical Association (AMA)
Boros, Éva& Kellermayer, Zoltán& Balogh, Péter& Strifler, Gerda& Vörös, Andrea& Sarlós, Patrícia…[et al.]. Elevated Expression of AXL May Contribute to the Epithelial-to-Mesenchymal Transition in Inflammatory Bowel Disease Patients. Mediators of Inflammation. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1203539
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1203539